Institute of Molecular Medicine, Division of Pathobiochemistry, Martin-Luther-University of Halle-Wittenberg, D-06114 Halle, Germany.
Biol Chem. 2013 Aug;394(8):1005-17. doi: 10.1515/hsz-2013-0114.
P0071 is a member of a subfamily of armadillo proteins that also comprises p120-catenin (p120ctn), δ-catenin/NPRAP, ARVCF and the more distantly related plakophilins 1-3. These proteins share a conserved central domain consisting of a series of repeated motifs, the armadillo repeats, which is flanked by more diverse amino- and carboxy-terminal domains. P0071 and the related proteins were first described as components of adherens junctions with a function in clustering and stabilizing cadherins, thereby controlling intercellular adhesion. In addition, these proteins show a cytoplasmic and a nuclear localization. Major progress in understanding their cytoplasmic role has been made in recent years. One common theme appears to be the spatiotemporal control of the small GTPases of the Rho family in various cellular contexts, such as cell adhesion and motility, cell division or neurite outgrowth. In this review article, we focus on the functions of the p0071 protein and its closest relatives in regulating cell adhesion and cytoskeletal organization, which are critically involved in the control of cell polarity. Understanding p0071's multiple functions requires assigning specific functions to particular binding partners and subcellular compartments. The identification of several new p0071 interacting proteins has promoted our understanding of the complex functions of this protein. Moreover, an initial analysis of its regulation begins to shed light on how these functions are coordinated in a cellular context.
P0071 是一种被称为“装甲蛋白亚家族”的成员之一，该亚家族还包括 p120-连环蛋白（p120ctn）、δ-连环蛋白/NPRAP、ARVCF 和更远亲的 plakophilins1-3。这些蛋白共享一个保守的中央结构域，该结构域由一系列重复的基序组成，称为“装甲重复序列”，其两侧为更具多样性的氨基和羧基末端结构域。P0071 和相关蛋白最初被描述为黏着连接的组成部分，在聚集和稳定钙黏蛋白方面发挥作用，从而控制细胞间黏附。此外，这些蛋白还具有细胞质和核定位。近年来，人们在理解其细胞质功能方面取得了重大进展。一个共同的主题似乎是在各种细胞环境中，时空控制 Rho 家族的小 GTPases，例如细胞黏附和运动、细胞分裂或神经突生长。在这篇综述文章中，我们重点介绍 P0071 蛋白及其最密切相关蛋白在调节细胞黏附和细胞骨架组织中的功能，这些功能对于控制细胞极性至关重要。理解 P0071 的多种功能需要将特定的功能分配给特定的结合伴侣和亚细胞区室。鉴定出几种新的 P0071 相互作用蛋白，促进了我们对该蛋白复杂功能的理解。此外，对其调节的初步分析开始揭示这些功能如何在细胞环境中协调。
