Laboratorio de Parasitología, Centro de Investigaciones Agrarias de Mabegondo, Instituto Galego da Calidade Alimentaria-Xunta de Galicia, Carretera AC-542 de Betanzos a Mesón do Vento, Km 7, CP 15318, Abegondo, A Coruña, Spain.
Vet Parasitol. 2013 Sep 23;196(3-4):497-502. doi: 10.1016/j.vetpar.2013.04.017. Epub 2013 Apr 18.
The aim of the present study was to identify the species of Cryptosporidium infecting Eurasian wild boars (Sus scrofa) in Galicia (NW, Spain). A sampling of 209 wild boars shot in different game preserves was carried out during the hunting season in 2009-2010. All samples were examined for Cryptosporidium infection, using both immunological and molecular tools. Cryptosporidium oocysts in faecal samples were identified using a direct immunofluorescence technique with monoclonal antibodies (DFA). The presence of Cryptosporidium DNA was determined using nested PCR involving amplification of a fragment of the small-subunit (SSU) ribosomal RNA gene (SSU rRNA). A total of 35 (16.7%) samples tested positive with both techniques. However, sequencing was only possible in 27 samples. Cryptosporidium scrofarum, Cryptosporidium suis and Cryptosporidium parvum oocysts were identified in 19, 5 and 3 of the samples, respectively. Moreover, C. scrofarum was detected as a dominant species infecting all age groups (juveniles, sub adults and adults). Sequence analyses of the glycoprotein (GP60) gene revealed the presence of C. parvum subtypes IIaA16G2R1 in 2 juveniles and IIaA13G1R1 in 1 sub adult wild boar. These species and subtypes have previously been described in human patients, indicating that isolates from asymptomatic wild boars might have zoonotic potential. This is the first report of the presence of C. scrofarum, C. suis and C. parvum subtypes IIaA16G2R1 and IIaA13G1R1 in wild boars (S. scrofa) in Spain.
本研究旨在鉴定感染西班牙加利西亚（西北部）野猪（Sus scrofa）的隐孢子虫物种。在 2009-2010 年狩猎季节，对不同狩猎保护区中捕获的 209 头野猪进行了采样。使用免疫和分子工具检查所有样本是否存在隐孢子虫感染。使用单克隆抗体（DFA）的直接免疫荧光技术鉴定粪便样本中的隐孢子虫卵囊。使用涉及扩增小亚基（SSU）核糖体 RNA 基因（SSU rRNA）片段的巢式 PCR 确定隐孢子虫 DNA 的存在。共有 35 个（16.7％）样本两种技术均呈阳性。但是，仅对 27 个样本进行了测序。在 19 个样本中鉴定出隐孢子虫 scrofarum、隐孢子虫 suis 和隐孢子虫 parvum 卵囊，在 5 个和 3 个样本中分别鉴定出 5 个和 3 个卵囊。此外，C. scrofarum 被检测为感染所有年龄段（幼崽、亚成年和成年）的主要物种。糖蛋白（GP60）基因序列分析显示，2 头幼崽中存在隐孢子虫 parvum 亚型 IIaA16G2R1，1 头亚成年野猪中存在 IIaA13G1R1。这些物种和亚型以前在人类患者中已有描述，表明来自无症状野猪的分离株可能具有人畜共患潜力。这是首次在西班牙野猪（S. scrofa）中报告存在 C. scrofarum、C. suis 和 C. parvum 亚型 IIaA16G2R1 和 IIaA13G1R1。
