Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
Biosens Bioelectron. 2013 Oct 15;48:12-8. doi: 10.1016/j.bios.2013.03.069. Epub 2013 Apr 4.
A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5h, and their ALP activity in a glucose-free buffer decreased after 2h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3h. The chip is expected to be useful for the evaluation of cell activities.
一种基于大规模集成电路 (LSI) 的电流传感器用于对碱性磷酸酶 (ALP) 活性进行电化学评估和实时监测。胚胎体 (EB) 通过胚胎干细胞 (ES) 的悬滴培养制备。在生物 LSI 芯片上的 400 个测量点处对具有不同大小的 EB 的 ALP 活性进行电化学检测。电化学测量结果表明，大 EB 的相对 ALP 活性较低，并且随着 ES 细胞分化水平的提高而降低。成功地对 EB 进行了 3.5 小时的实时监测，并且在 2 小时后它们在无葡萄糖缓冲液中的 ALP 活性降低。据我们所知，这是首次应用基于 LSI 的电流传感器对 3 小时以上的实时细胞监测的报道。该芯片有望用于评估细胞活性。
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