Ozawa Fumisato, Ino Kosuke, Shiku Hitoshi, Matsue Tomokazu
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8579, Japan.
Materials (Basel). 2016 Aug 31;9(9):744. doi: 10.3390/ma9090744.
Here we propose a novel electrochemical lithography methodology for fabricating calcium-alginate hydrogels having controlled shapes. We separated the chambers for Ca production and gel formation with alginate with a semipermeable membrane. Ca formed in the production chamber permeated through the membrane to fabricate a gel structure on the membrane in the gel formation chamber. When the calcium-alginate hydrogels were modified with collagen, HepG2 cells proliferated on the hydrogels. These results show that electrochemical hydrogel lithography is useful for cell culture.
在此，我们提出了一种用于制造具有可控形状的海藻酸钙水凝胶的新型电化学光刻方法。我们用半透膜将用于钙生成的腔室与用于海藻酸凝胶形成的腔室隔开。在生成腔室中形成的钙透过膜渗透，从而在凝胶形成腔室的膜上制造凝胶结构。当用胶原蛋白修饰海藻酸钙水凝胶时，HepG2细胞在水凝胶上增殖。这些结果表明，电化学水凝胶光刻技术对细胞培养有用。
