Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain.
Dev Comp Immunol. 2013 Sep;41(1):100-4. doi: 10.1016/j.dci.2013.04.020. Epub 2013 May 3.
In this report we employed laser-capture microdissection (LCM) coupled to qPCR technology and bioinformatic analysis to characterize, for the first time, the response of Peyer's patches (PP) from orally infected animals to Salmonella typhimurium, in a model of non-typhoidal salmonellosis. Pathogen was highly found in the cytoplasm of phagocytes in PP and differential gene expression analysis indicated an up-regulation of proinflammatory molecules, establishment of a Th1 driven response and triggering of DC and T-cell activity. Furthermore, predictions by bioinformatic analysis pointed to an activation of processes regarding stimulation and maturation of DC, influx of leukocytes in tissue and T lymphocytes priming and differentiation. In short, the approach used in this study proved to be a promising strategy to explore infectious processes. Indeed, it revealed an effective induction of innate and adaptive immune mechanisms in swine PP which appear to be distinct from those observed in mesenteric lymph nodes and closely related to response of gut mucosa.
在本报告中，我们首次采用激光捕获显微切割（LCM）结合 qPCR 技术和生物信息学分析，来研究非伤寒沙门氏菌感染模型中，肠道派尔集合淋巴结（PP）对鼠伤寒沙门氏菌的反应。病原体在 PP 中的吞噬细胞胞质中高度存在，差异基因表达分析表明促炎分子的上调、Th1 驱动反应的建立以及 DC 和 T 细胞活性的触发。此外，生物信息学分析的预测指出，DC 的刺激和成熟、白细胞在组织中的浸润以及 T 淋巴细胞的启动和分化等过程被激活。简而言之，本研究中使用的方法被证明是探索感染过程的有前途的策略。事实上，它揭示了猪 PP 中固有和适应性免疫机制的有效诱导，这些机制似乎与肠系膜淋巴结观察到的不同，与肠道黏膜的反应密切相关。
