Javed Talha, Zhou Jing-Ru, Li Juan, Hu Zhong-Ting, Wang Qin-Nan, Gao San-Ji
National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou, China.
Front Plant Sci. 2022 Jun 9;13:917953. doi: 10.3389/fpls.2022.917953. eCollection 2022.
WRKY transcription factors (TFs) are essential players in different signaling cascades and regulatory networks involved in defense responses to various stressors. This study systematically analyzed and characterized WRKY family genes in the spp. hybrid R570 and their expression in two sugarcane cultivars LCP85-384 (resistant to leaf scald) and ROC20 (susceptible to leaf scald) in response to bacterial pathogen infection and nitrogen implantation dosage. A total of 53 genes with 66 alleles were systematically identified in R570 based on the query sequence in AP85-441. All alleles were further classified into four groups with 11 (16.7%) genes in group I, 36 (54.5%) genes in group II, 18 (27.3%) genes in group III, and 1 (1.5%) gene in group IV. Among them, 4 and 11 gene pairs displayed tandem and segmental duplication events, respectively. The genes exhibited conserved DNA-binding domains, which were accompanied by variations in introns, exons, and motifs. RT-qPCR analysis of two sugarcane cultivars triggered by () revealed that four genes, , exhibited significant upregulation in leaf scald-resistant LCP85-384. These WRKY genes were downregulated or unchanged in ROC20 at 24-72 h post-inoculation, suggesting that they play an important role in defense responses to infection. Most of the 12 tested s, in particular, functioned as negative regulators in the two cultivars in response to a range of nitrogen (N) implantation doses. A total of 11 ShWRKY proteins were predicted to interact with each other. ShWRKY43 and ShWRKY49-3 are predicted to play core roles in the interaction network, as indicated by their interaction with six other ShWRKY proteins. Our results provide important candidate gene resources for the genetic improvement of sugarcane and lay the foundation for further functional characterization of genes in response to coupling effects of infection and different N levels.
WRKY转录因子（TFs）是参与对各种应激源防御反应的不同信号级联和调控网络中的关键因子。本研究系统分析并鉴定了 spp. 杂交种R570中的WRKY家族基因，以及它们在两个甘蔗品种LCP85 - 384（抗叶烧病）和ROC20（感叶烧病）中对细菌病原体感染和氮注入剂量的响应表达情况。基于AP85 - 441中的查询序列，在R570中系统鉴定出了总共53个具有66个等位基因的基因。所有等位基因进一步分为四组，第一组有11个（16.7%）基因，第二组有36个（54.5%）基因，第三组有18个（27.3%）基因，第四组有1个（1.5%）基因。其中，分别有4对和11对基因显示出串联和片段重复事件。这些基因表现出保守的DNA结合结构域，同时内含子、外显子和基序存在变异。对两个甘蔗品种由（）引发的RT - qPCR分析表明，四个基因，在抗叶烧病的LCP85 - 384中显著上调。在接种后24 - 72小时，这些WRKY基因在ROC20中下调或未改变，表明它们在对感染的防御反应中起重要作用。在12个测试的中，大多数，特别是，在两个品种中对一系列氮（N）注入剂量起负调控作用。总共预测有11个ShWRKY蛋白相互作用。预测ShWRKY43和ShWRKY49 - 3在相互作用网络中起核心作用，这通过它们与其他六个ShWRKY蛋白的相互作用得以表明。我们的结果为甘蔗的遗传改良提供了重要的候选基因资源，并为进一步研究基因在响应感染和不同氮水平耦合效应时的功能特性奠定了基础。
