Co-Innovation Center for Sustainable Forestry in Southern China; College of Forestry, Nanjing Forestry University, Nanjing, China.
Plant Signal Behav. 2021 Oct 3;16(10):1930442. doi: 10.1080/15592324.2021.1930442. Epub 2021 May 23.
WRKY transcription factors are important regulators of diverse plant life processes. Our aim was to clone and characterize , a WRKY gene of group IIc, derived from . The cDNA sequence of was 818 bp long, encoding a 271-amino acid proteins and containing two introns and three exons. The proteinic molecular weight was 30.99 kDa, with a relevant theoretical isoelectric point of 8.15. Subcellular localization analysis confirmed that the protein localized to the nucleus. In total, 75 cis-regulatory elements of 19 different types were identified in the promoter sequence, including some elements involved in light responsiveness, anaerobic induction and circadian control, low-temperature responsiveness, as well as salicylic acid (SA) and auxin responsiveness. Expression pattern analysis of plant samples from different developmental stages and tissue types, revealed differential expression. The transcript was downregulated 12 h after heat treatment and at 4-12 h after drought treatment, but was upregulated 12 h after NaCl, cold and methyl jasmonate treatments. For abscisic acid and SA treatments, the transcript was upregulated at 24 h. In summary, encoded a newly cloned WRKY transcription factor of that might be involved in plant growth and plant responses to abiotic stresses and hormones treatments.
WRKY 转录因子是植物多种生命过程的重要调控因子。本研究旨在从 中克隆和鉴定一个属于 IIc 组的 WRKY 基因， 。 的 cDNA 序列长 818bp，编码一个 271 个氨基酸的蛋白质，包含两个内含子和三个外显子。该蛋白质的分子量为 30.99kDa，理论等电点为 8.15。亚细胞定位分析证实 蛋白定位于细胞核。在 启动子序列中共鉴定出 19 种不同类型的 75 个顺式调控元件，包括一些与光响应、厌氧诱导和昼夜节律控制、低温响应以及水杨酸（SA）和生长素响应相关的元件。对不同发育阶段和组织类型的植物样本进行表达模式分析，揭示了 的差异表达。热处理 12 小时后，干旱处理 4-12 小时后， 转录本下调，但 NaCl、冷和茉莉酸甲酯处理 12 小时后上调。对于脱落酸和 SA 处理，24 小时后 转录本上调。总之， 编码了一个新克隆的属于 IIc 组的 WRKY 转录因子，可能参与植物生长以及植物对非生物胁迫和激素处理的响应。
