Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, USA.
Nat Genet. 2013 Jun;45(6):656-63. doi: 10.1038/ng.2625. Epub 2013 May 5.
Whole-genome sequencing of 616 asymptomatically carried Streptococcus pneumoniae isolates was used to study the impact of the 7-valent pneumococcal conjugate vaccine. Comparison of closely related isolates showed the role of transformation in facilitating capsule switching to non-vaccine serotypes and the emergence of drug resistance. However, such recombination was found to occur at significantly different rates across the species, and the evolution of the population was primarily driven by changes in the frequency of distinct genotypes extant before the introduction of the vaccine. These alterations resulted in little overall effect on accessory genome composition at the population level, contrasting with the decrease in pneumococcal disease rates after the vaccine's introduction.
对 616 株无症状携带肺炎链球菌分离株进行全基因组测序，以研究 7 价肺炎球菌结合疫苗的影响。对密切相关的分离株进行比较，表明转化在促进荚膜向非疫苗血清型转换以及耐药性出现方面的作用。然而，这种重组在种间的发生率存在显著差异，种群的进化主要是由疫苗引入前存在的不同基因型的频率变化驱动的。这些改变导致在种群水平上，附属基因组组成几乎没有总体影响，与疫苗引入后肺炎球菌病发病率下降形成对比。
