Institute of Bioengineering, School of Engineering and School of Life Science, École Polytechnique Fédérale de Lausanne, Switzerland.
Nat Methods. 2013 Jun;10(6):550-2. doi: 10.1038/nmeth.2473. Epub 2013 May 5.
High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We developed a simple yet powerful method that uses contact spotting to generate high-density nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries.
高内涵分析有潜力极大地提高细胞生物学和药物发现的通量，但是处理和培养大量的细胞库，如原代肿瘤或癌细胞系，需要昂贵的、专用的机器人设备。我们开发了一种简单而强大的方法，使用接触点样来生成高密度的活哺乳动物细胞纳米孔阵列，用于细胞文库的培养和检测。
