Department of Natural Resources, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Safir-e-Sabz Co. Isfahan Science & Technology Town, Isfahan, 84156, Iran.
J Hazard Mater. 2013 Jun 15;254-255:372-381. doi: 10.1016/j.jhazmat.2013.03.004. Epub 2013 Apr 2.
Bioremediation is a promising technique for reclamation of oil polluted soils. In this study, six methods for enhancing bioremediation were tested on oil contaminated soils from three refinery areas in Iran (Isfahan, Arak, and Tehran). The methods included bacterial enrichment, planting, and addition of nitrogen and phosphorous, molasses, hydrogen peroxide, and a surfactant (Tween 80). Total petroleum hydrocarbon (TPH) concentrations and CHEMometric analysis of Selected Ion Chromatograms (SIC) termed CHEMSIC method of petroleum biomarkers including terpanes, regular, diaromatic and triaromatic steranes were used for determining the level and type of hydrocarbon contamination. The same methods were used to study oil weathering of 2 to 6 ring polycyclic aromatic compounds (PACs). Results demonstrated that bacterial enrichment and addition of nutrients were most efficient with 50% to 62% removal of TPH. Furthermore, the CHEMSIC results demonstrated that the bacterial enrichment was more efficient in degradation of n-alkanes and low molecular weight PACs as well as alkylated PACs (e.g. C₃-C₄ naphthalenes, C₂ phenanthrenes and C₂-C₃ dibenzothiophenes), while nutrient addition led to a larger relative removal of isoprenoids (e.g. norpristane, pristane and phytane). It is concluded that the CHEMSIC method is a valuable tool for assessing bioremediation efficiency.
生物修复是一种很有前途的技术，可用于受污染土壤的修复。在这项研究中，测试了六种增强生物修复的方法，这些方法用于处理来自伊朗三个炼油区（伊斯法罕、阿拉克和德黑兰）的污染土壤。这些方法包括细菌富集、种植以及添加氮和磷、糖蜜、过氧化氢和表面活性剂（吐温 80）。总石油烃（TPH）浓度和选定离子色谱图（SIC）的化学计量分析（称为石油生物标志物的 CHEMSIC 方法）包括萜烷、规则甾烷、二芳甾烷和三芳甾烷，用于确定烃污染的程度和类型。同样的方法也用于研究 2 到 6 个环多环芳烃（PACs）的油风化。结果表明，细菌富集和添加营养物质的效率最高，可去除 TPH 的 50%至 62%。此外， CHEMSIC 结果表明，细菌富集在降解正烷烃和低分子量 PACs 以及烷基化 PACs（例如 C₃-C₄萘、C₂菲和 C₂-C₃二苯并噻吩）方面更有效，而营养物质的添加导致异戊二烯（例如，姥鲛烷、植烷和姥烷）的相对去除率更高。因此，可以得出结论， CHEMSIC 方法是评估生物修复效率的一种有价值的工具。
