Ebadi Ali, Khoshkholgh Sima Nayer Azam, Olamaee Mohsen, Hashemi Maryam, Ghorbani Nasrabadi Reza
Department of Soil Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Agricultural Biotechnology Research Institute of Iran (ABRII), AREEO, P.O. Box: 31535-1897, Karaj, Iran.
J Adv Res. 2017 Nov;8(6):627-633. doi: 10.1016/j.jare.2017.06.008. Epub 2017 Jun 29.
Bacteria able to produce biosurfactants can use petroleum-based hydrocarbons as a carbon source. Herein, four biosurfactant-producing strains, isolated from oil-contaminated saline soil, were combined to form a bacterial consortium. The inoculation of the consortium to contaminated soil alleviated the adverse effects of salinity on biodegradation and increased the rate of degradation of petroleum hydrocarbon approximately 30% compared to the rate achieved in non-treated soil. In saline condition, treatment of polluted soil with the consortium led to a significant boost in the activity of dehydrogenase (approximately 2-fold). A lettuce seedling bioassay showed that, following the treatment, the soil's level of phytotoxicity was reduced up to 30% compared to non-treated soil. Treatment with an appropriate bacterial consortium can represent an effective means of reducing the adverse effects of salinity on the microbial degradation of petroleum and thus provides enhancement in the efficiency of microbial remediation of oil-contaminated saline soils.
能够产生生物表面活性剂的细菌可以利用石油基碳氢化合物作为碳源。在此，从受石油污染的盐渍土壤中分离出的四株产生物表面活性剂菌株被组合形成一个细菌联合体。将该联合体接种到受污染土壤中，减轻了盐分对生物降解的不利影响，与未处理土壤相比，石油烃降解率提高了约30%。在盐渍条件下，用该联合体处理污染土壤导致脱氢酶活性显著提高（约2倍）。生菜幼苗生物测定表明，处理后，与未处理土壤相比，土壤的植物毒性水平降低了30%。用合适的细菌联合体进行处理可以成为减少盐分对石油微生物降解不利影响的有效手段，从而提高石油污染盐渍土壤微生物修复的效率。
