Laboratoire de Recherche (LR11-ES41): Génétique, Biodiversité et Valorisation des Bio-ressources, Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Monastir, Tunisia,
Environ Sci Pollut Res Int. 2013 Oct;20(10):7309-19. doi: 10.1007/s11356-013-1745-3. Epub 2013 May 5.
The impact of heavy metal levels (Cd, Cu, and Zn) on the reproductive parameters in natural populations of the Mediterranean killifish Aphanius fasciatus was monitored in four sites of the Tunisian coast. Our study covered a period of the reproductive cycle, from April to November 2009. The significantly higher levels of Cd, Cu, and Zn in the liver and gonads for both sexes (p<0.05) were observed in the most polluted site (Sfax coast). During the reproductive cycle of A. fasciatus, an unbalanced sex ratio in favor to females was observed for all the studied populations. The progression of the hepato- (HSI), gonadosomatic (GSI), and K-factor revealed a spatio-temporal variation between populations during the sampling period. Estradiol-17β amounts in the gonad tissues of females captured from the studied sites showed a significant difference, and the highest concentrations were noticed in the control site (Luza). Taking together, our data provide the first evidence of a physiologically stress and a disturbance of the reproductive status in natural populations of A. fasciatus.
重金属水平（Cd、Cu 和 Zn）对地中海腹脂鲤自然种群生殖参数的影响在突尼斯海岸的四个地点进行了监测。我们的研究涵盖了 2009 年 4 月至 11 月的生殖周期。在污染最严重的地点（斯法克斯海岸），观察到两性肝和性腺中 Cd、Cu 和 Zn 的含量明显更高（p<0.05）。在腹脂鲤的生殖周期中，所有研究种群的雌雄性别比例失衡，雌性占优势。在采样期间，肝体比（HSI）、性腺体比（GSI）和 K 因子的进展在种群之间表现出时空变化。从研究地点捕获的雌性性腺组织中的雌二醇-17β含量存在显著差异，最高浓度出现在对照点（卢扎）。总之，我们的数据首次提供了证据，表明腹脂鲤自然种群存在生理应激和生殖状态紊乱。
