Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS), 1 Avenue de l'Agrobiopole BP 32607 Auzeville Tolosane, 31326 Castanet-Tolosan, France.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3744-3749. doi: 10.1099/ijs.0.051839-0. Epub 2013 May 3.
The taxonomic position of a novel actinomycete, strain SA152(T), isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The strain produced abundant aerial mycelium and fragmented substrate mycelium on most media tested. Chemotaxonomically and phylogenetically, the strain was related to the members of the genus Saccharothrix. Results of 16S rRNA gene sequence comparison revealed that strain SA152(T) shared the highest degree of 16S rRNA gene sequence similarity with Saccharothrix xinjiangensis NBRC 101911(T) (99.3 %) and Saccharothrix texasensis NRRL B-16134(T) (98.9 %). However, DNA-DNA hybridization studies showed only 16.2 % relatedness with S. xinjiangensis DSM 44896(T) and 33.9 % relatedness with S. texasensis DSM 44231(T). Based upon genotypic and phenotypic differences from other members of the genus, a novel species, Saccharothrix saharensis sp. nov., is proposed, with SA152(T) ( = DSM 45456(T) = CCUG 60213(T)) as the type strain.
从阿尔及利亚撒哈拉土壤样本中分离出的新型放线菌菌株 SA152(T)，通过多相分类学方法确定了其分类地位。该菌株在大多数测试的培养基上产生丰富的气生菌丝和断裂的基质菌丝。化学分类学和系统发育分析表明，该菌株与糖丝菌属的成员密切相关。16S rRNA 基因序列比较结果表明，SA152(T)与新疆糖丝菌 NBRC 101911(T)（99.3%）和德克萨斯糖丝菌 NRRL B-16134(T)（98.9%）的 16S rRNA 基因序列相似度最高。然而，DNA-DNA 杂交研究表明，与新疆糖丝菌 DSM 44896(T)的相关性仅为 16.2%，与德克萨斯糖丝菌 DSM 44231(T)的相关性为 33.9%。基于与属内其他成员的基因型和表型差异，提出了一个新的物种，即撒哈拉糖丝菌，SA152(T)（=DSM 45456(T)=CCUG 60213(T)）作为模式菌株。
