Bouznada Khaoula, Bouras Noureddine, Mokrane Salim, Chaabane Chaouch Fawzia, Zitouni Abdelghani, Pötter Gabriele, Spröer Cathrin, Klenk Hans-Peter, Sabaou Nasserdine
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, 47000, Ghardaïa, Algeria.
Antonie Van Leeuwenhoek. 2017 Mar;110(3):399-405. doi: 10.1007/s10482-016-0812-4. Epub 2016 Dec 3.
The taxonomic position of a new Saccharothrix strain, designated MB46, isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria) was established following a polyphasic approach. The novel microorganism has morphological and chemical characteristics typical of the members of the genus Saccharothrix and formed a phyletic line at the periphery of the Saccharothrix espanaensis subcluster in the 16S rRNA gene dendrograms. Results of the 16S rRNA gene sequence comparisons revealed that strain MB46 shares high degrees of similarity with S. espanaensis DSM 44229 (99.2%), Saccharothrix variisporea DSM 43911 (98.7%) and Saccharothrix texasensis NRRL B-16134 (98.6%). However, the new strain exhibited only 12.5-17.5% DNA relatedness to the neighbouring Saccharothrix spp. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, strain MB46 is concluded to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix ghardaiensis sp. nov. (type strain MB46 = DSM 46886 = CECT 9046) is proposed.
采用多相分类法确定了从阿尔及利亚南部加尔达亚省姆扎卜地区采集的撒哈拉土壤样本中分离出的一株新的糖丝菌属菌株（命名为MB46）的分类地位。该新型微生物具有糖丝菌属成员典型的形态和化学特征，并且在16S rRNA基因树状图中位于西班牙糖丝菌亚簇的边缘形成一个进化系。16S rRNA基因序列比较结果显示，菌株MB46与西班牙糖丝菌DSM 44229（相似度99.2%）、多变糖丝菌DSM 43911（相似度98.7%）和得克萨斯糖丝菌NRRL B - 16134（相似度98.6%）具有高度相似性。然而，该新菌株与相邻的糖丝菌属物种的DNA相关性仅为12.5 - 17.5%。基于表型特征、16S rRNA基因序列比较和DNA - DNA杂交结果，得出菌株MB46代表糖丝菌属一个新物种的结论，为此提出名为加尔达亚糖丝菌（Saccharothrix ghardaiensis sp. nov.）（模式菌株MB46 = DSM 46886 = CECT 9046）。
