Plucani do Amaral Fernanda, Wang Juexin, Williams Jacob, Tuleski Thalita R, Joshi Trupti, Ferreira Marco A R, Stacey Gary
Divisions of Plant Sciences and Technology, C. S. Bond Life Science Center, University of Missouri, Columbia, MO 65211, USA.
Ginkgo Bioworks, Ag Biologicals, 890 Embarcadero Dr., West Sacramento, CA 95605, USA.
Microorganisms. 2023 Jan 28;11(2):331. doi: 10.3390/microorganisms11020331.
Plant growth-promoting bacteria (PGPB) can enhance plant health by facilitating nutrient uptake, nitrogen fixation, protection from pathogens, stress tolerance and/or boosting plant productivity. The genetic determinants that drive the plant-bacteria association remain understudied. To identify genetic loci highly correlated with traits responsive to PGPB, we performed a genome-wide association study (GWAS) using an population treated with DQS-4. Phenotypically, the 305 Arabidopsis accessions tested responded differently to bacterial treatment by improving, inhibiting, or not affecting root system or shoot traits. GWA mapping analysis identified several predicted loci associated with primary root length or root fresh weight. Two statistical analyses were performed to narrow down potential gene candidates followed by haplotype block analysis, resulting in the identification of 11 loci associated with the responsiveness of Arabidopsis root fresh weight to bacterial inoculation. Our results showed considerable variation in the ability of plants to respond to inoculation by DQS-4 while revealing considerable complexity regarding statistically associated loci with the growth traits measured. This investigation is a promising starting point for sustainable breeding strategies for future cropping practices that may employ beneficial microbes and/or modifications of the root microbiome.
植物促生细菌（PGPB）可通过促进养分吸收、固氮、抵御病原体、增强胁迫耐受性和/或提高植物生产力来增强植物健康。驱动植物与细菌关联的遗传决定因素仍未得到充分研究。为了鉴定与对PGPB响应性状高度相关的基因座，我们使用经DQS - 4处理的群体进行了全基因组关联研究（GWAS）。从表型上看，所测试的305份拟南芥种质对细菌处理的反应不同，表现为改善、抑制或不影响根系或地上部性状。全基因组关联图谱分析确定了几个与主根长度或根鲜重相关的预测基因座。进行了两项统计分析以缩小潜在基因候选范围，随后进行单倍型块分析，从而确定了11个与拟南芥根鲜重对细菌接种反应性相关的基因座。我们的结果表明，植物对接种DQS - 4的反应能力存在相当大的差异，同时揭示了与所测量生长性状的统计相关基因座具有相当大的复杂性。这项研究是未来种植实践可持续育种策略的一个有前景的起点，这些种植实践可能会利用有益微生物和/或对根际微生物群进行改造。
