Sha Baoyong, Gao Wei, Han Yulong, Wang ShuQi, Wu Jinhui, Xu Feng, Lu TianJian
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
J Nanosci Nanotechnol. 2013 Feb;13(2):1208-11. doi: 10.1166/jnn.2013.6081.
Osteosarcoma and chondrosarcoma are malignant bone tumors, and they significantly affect the life quality of patients including children and adults. The main treatment method is surgical amputation of the malignant lesion, despite that recurrence often occurs. Recently, it has been observed that TiO2 NPs killed HeLa cells effectively via photocatalysis in vitro, which indicates titanium dioxide (TiO2) nanoparticles (NPs) might be used to reduce the recurrence of osteosarcoma and chondrosarcoma by inducing cytotoxicity to bone tumor cells. In this study, we investigated the potential effects of TiO2 NPs in two cancer cell lines in vitro: U-2 OS (osteosarcoma) and SW 1353 (chondrosarcoma). We assessed cell viability, the levels of reactive oxygen species (ROS) and glutathione (GSH) after exposure to TiO2 NPs at different concentrations (0.1-100 microg/ml) for varying exposure periods (12-48 hours). Compared to the NP-free control, TiO2 NPs induced cell death in a dosage-dependent and time-dependent manner. The median inhibitory concentration (IC50) of TiO2 NPs at 24 hours was 211.3 +/- 15.2 microg/ml and 5408.8 +/- 45.9 microg/ml for SW 1353 and U-2 OS cell lines, respectively. TiO2 NPs concentrations above 1 microg/ml were more efficient to reduce the cell viability of SW 1353 than U-2 OS of NPs at all exposure times. The increased ROS and reduced GSH levels indicated that TiO2 NPs killed cancer cells through oxidative stress. These results suggested that the TiO2 NPs can be potentially used to minimize/prevent the recurrence of osteosarcoma and chondrosarcoma.
骨肉瘤和软骨肉瘤是恶性骨肿瘤，它们严重影响包括儿童和成人在内的患者的生活质量。尽管经常会复发，但主要的治疗方法仍是对恶性病变进行手术截肢。最近，有研究观察到二氧化钛纳米颗粒（TiO2 NPs）在体外通过光催化作用可有效杀死宫颈癌细胞，这表明二氧化钛（TiO2）纳米颗粒可能通过诱导对骨肿瘤细胞的细胞毒性来降低骨肉瘤和软骨肉瘤的复发率。在本研究中，我们在体外研究了TiO2 NPs对两种癌细胞系的潜在影响：U-2 OS（骨肉瘤）和SW 1353（软骨肉瘤）。我们评估了在不同浓度（0.1 - 100微克/毫升）的TiO2 NPs作用不同暴露时间（12 - 48小时）后细胞的活力、活性氧（ROS）水平和谷胱甘肽（GSH）水平。与无NP对照组相比，TiO2 NPs以剂量和时间依赖性方式诱导细胞死亡。在24小时时，TiO2 NPs对SW 1353和U-2 OS细胞系的半数抑制浓度（IC50）分别为211.3±15.2微克/毫升和5408.8±45.9微克/毫升。在所有暴露时间，浓度高于1微克/毫升的TiO2 NPs对SW 1353细胞活力的降低作用比U-2 OS细胞更有效。ROS水平升高和GSH水平降低表明TiO2 NPs通过氧化应激杀死癌细胞。这些结果表明，TiO2 NPs有可能用于最小化/预防骨肉瘤和软骨肉瘤的复发。
