Diaspro A, Adami M, Sartore M, Nicolini C
Institute of Biophysics, School of Medicine, University of Genova, Italy.
Comput Methods Programs Biomed. 1990 Mar-Apr;31(3-4):225-36. doi: 10.1016/0169-2607(90)90007-v.
This work describes IMAGO, an integrated bio-imaging system developed in our laboratory. The whole system consists of a personal computer, a commercially available frame grabber directly plugged into a personal computer, video input/output modules, specific hardware for z-axis movement and light shuttering, and a software package. IMAGO is user-friendly, menu driven and enables one to perform image acquisition with different methods: optical sectioning, flashing epifluorescence, transmitted and phase contrast microscopy. It makes various functions possible, including: image transfer, gray scale processing, conventional and advanced filtering, logical operations, look-up table management, three-dimensional (3D) editing, 3D representation and auto-correlation techniques. More than 100 image processing functions have been implemented and can be easily managed through IMAGO. Examples are given in the area of biophysical research, like 3D representation of nuclei and of electron microscopic images, in situ microscopy of living cells. IMAGO processes information in an x, y, z, t space.
这项工作描述了IMAGO，这是我们实验室开发的一个集成生物成像系统。整个系统由一台个人计算机、一个直接插入个人计算机的商用图像采集卡、视频输入/输出模块、用于z轴移动和光闸控制的特定硬件以及一个软件包组成。IMAGO用户友好，由菜单驱动，能让用户使用不同方法进行图像采集：光学切片、闪光落射荧光、透射和相差显微镜观察。它实现了各种功能，包括：图像传输、灰度处理、常规和高级滤波、逻辑运算、查找表管理、三维（3D）编辑、3D表示和自相关技术。已经实现了100多种图像处理功能，并且可以通过IMAGO轻松管理。在生物物理研究领域给出了一些例子，如细胞核和电子显微镜图像的3D表示、活细胞的原位显微镜观察。IMAGO在x、y、z、t空间中处理信息。
