Department of Oral & Cranio-maxillofacial Science, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Carbohydr Polym. 2013 Jun 20;95(2):644-8. doi: 10.1016/j.carbpol.2013.03.035. Epub 2013 Mar 15.
Process conditions (extraction time, extraction temperature, water/solid ratio and incubation time) of Shiitake mushroom polysaccharides (SMP) were optimized by conducting experiments at three different levels using the response surface method (RSM). A second-order polynomial response surface equation was developed indicating the effect of variables on polysaccharides yield. Contour maps generated using the response surface equation showed that all the experimental variables significantly affected the yield. The effect of SMP on oxidative damage in mice fed by high cholesterol diet (HCD) was done in vivo. Results showed that SMP can decreased serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-c) levels, and increased high density lipoprotein (HDL-c) levels in HCD mice. Treatment with SMP reduced blood, liver lipid peroxidation level and increased antioxidant enzymes activities. Thus it can be concluded that SMP can improve lipid metabolism and decreased oxidative damage in HCD mice.
采用响应面法（RSM）在三个不同水平上进行实验，对香菇多糖（SMP）的提取工艺条件（提取时间、提取温度、水/固比和孵育时间）进行了优化。建立了一个二阶多项式响应面方程，表明变量对多糖得率的影响。利用响应面方程生成的等高线图表明，所有实验变量都显著影响产率。体内实验研究了 SMP 对高胆固醇饮食（HCD）喂养小鼠氧化损伤的影响。结果表明，SMP 可降低 HCD 小鼠血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-c）水平，提高高密度脂蛋白（HDL-c）水平。SMP 处理可降低血液、肝脏脂质过氧化水平，提高抗氧化酶活性。因此，可以得出结论，SMP 可以改善 HCD 小鼠的脂质代谢，降低氧化损伤。
