Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
Carbohydr Polym. 2013 Jun 20;95(2):740-5. doi: 10.1016/j.carbpol.2013.03.002. Epub 2013 Mar 13.
In the present study, we firstly reported the antioxidant, antitumor and immunomodulatory effects of two polysaccharides (GUMP-1-1 and GUMP-1-2) isolated from Grifola umbellata mycelia. Chemical analysis indicated that two polysaccharide fractions contained different content of neutral sugar, uronic acid and protein, as well as varying monosaccharide compositions and average molecular weight. We found that they could significantly inhibit the growth of H22 implanted tumor and enhance the spleen index and splenocyte proliferation of H22 tumor-bearing mice. In addition, GUMP-1-2 had the stronger free radicals scavenging and ferrous ion chelating abilities than GUMP-1-1 in vitro. These results indicated that antitumor activity of two purified polysaccharides might be achieved by improving immune response and the different chemical composition and average molecular weight could affect their antitumor, antioxidant and immunomodulatory activities.
在本研究中，我们首次报道了从金针菇菌丝体中分离得到的两种多糖（GUMP-1-1 和 GUMP-1-2）的抗氧化、抗肿瘤和免疫调节作用。化学分析表明，两种多糖组分含有不同含量的中性糖、糖醛酸和蛋白质，以及不同的单糖组成和平均分子量。我们发现，它们能显著抑制 H22 移植瘤的生长，提高荷瘤小鼠的脾脏指数和脾淋巴细胞增殖。此外，GUMP-1-2 在体外具有比 GUMP-1-1 更强的清除自由基和螯合亚铁离子的能力。这些结果表明，两种纯化多糖的抗肿瘤活性可能是通过改善免疫反应实现的，而不同的化学组成和平均分子量可能会影响它们的抗肿瘤、抗氧化和免疫调节活性。
