College of Biosystem Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Xihu District, Hangzhou 310058, Zhejiang, China.
Food Funct. 2013 Apr 25;4(4):539-48. doi: 10.1039/c2fo30178d. Epub 2013 Jan 16.
The fresh water clam Corbicula fluminea is currently one of the most economically important aquatic species in China because of its nutritional value and pharmacological activity. In order to explore the potential of C. fluminea as a natural resource of bioactive compounds, a papain-released polysaccharide designated CFPS-2 was isolated. Chemical composition analysis indicated that CFPS-2 contained glucosamine, glucose, galactose, fucose, protein and sulfate groups, with an average molecular weight of about 22 kDa. Furthermore, the antioxidant and antitumor activities, in vitro, of the polysaccharide fractions (crude CFPS and purified CFPS-2) were evaluated. CFPS-2, which exhibited strong antioxidant activities in a dose dependent manner also showed significant inhibitory effects on growth of human gastric cancer cells (SGC7901) and human ovarian carcinoma cells (SKOV3 and A2780). The present results suggest that CFPS-2 could be a potential candidate for the development of novel functional food ingredient.
淡水贝类河蚬（Corbicula fluminea）是中国目前最具经济价值的水产养殖物种之一，这主要是因为其具有营养价值和药理学活性。为了探索河蚬作为生物活性化合物天然资源的潜力，本研究从河蚬中分离得到一种木瓜蛋白酶释放多糖，命名为 CFPS-2。化学组成分析表明，CFPS-2 含有氨基葡萄糖、葡萄糖、半乳糖、岩藻糖、蛋白质和硫酸基团，其平均分子量约为 22 kDa。此外，还评估了多糖组分（粗 CFPS 和纯化的 CFPS-2）的体外抗氧化和抗肿瘤活性。CFPS-2 具有剂量依赖性的强抗氧化活性，对人胃癌细胞（SGC7901）和人卵巢癌细胞（SKOV3 和 A2780）的生长也表现出显著的抑制作用。这些结果表明 CFPS-2 可能是开发新型功能性食品成分的潜在候选物。
