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首次从犊牛和成年牛中检测到巴西牛轮状病毒(BToV)毒株并分析其分子多样性。

First detection and molecular diversity of Brazilian bovine torovirus (BToV) strains from young and adult cattle.

机构信息

Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo. Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, Cidade Universitária, São Paulo, Brazil.

出版信息

Res Vet Sci. 2013 Oct;95(2):799-801. doi: 10.1016/j.rvsc.2013.04.006. Epub 2013 May 3.

DOI:10.1016/j.rvsc.2013.04.006
PMID:23648077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7111811/
Abstract

Bovine torovirus (BToV) is an established enteric pathogen of cattle, but its occurrence in Brazilian cattle had not been reported until now. This article describes a survey on BToV in Brazil carried out on 80 fecal samples from diarrheic young and adult cattle, using a nested-RT-PCR targeting the nucleocapsid (N) gene. BToV was detected in 6.25% (5/80) of stool samples from three different geographic regions. Sequences analysis showed that Brazilian BToVs have a high degree of identity with European and Japanese BToVs and a lower degree of identity with North American Breda 1 strain. These results show that, albeit its low frequency and the scarce number of research on the field, BToV is still present amongst cattle populations.

摘要

牛传染性冠状病毒(Bovine torovirus,BToV)是一种已被确认的牛肠道病原体,但直到现在,巴西牛群中的 BToV 感染情况仍未有报道。本文描述了一项针对巴西 BToV 的调查,共采集了 80 份来自腹泻幼牛和成年牛的粪便样本,使用巢式 RT-PCR 针对核衣壳(N)基因进行检测。结果显示,在来自三个不同地理区域的 5/80(6.25%)份粪便样本中检测到了 BToV。序列分析表明,巴西 BToVs 与欧洲和日本的 BToVs 具有高度的同源性,与北美的 Breda 1 株的同源性较低。这些结果表明,尽管 BToV 的流行率较低,且对其的研究也较少,但它仍存在于牛群中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1898/7111811/ac45abde4873/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1898/7111811/ac45abde4873/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1898/7111811/ac45abde4873/gr1.jpg

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