通过 S-腺苷-L-甲硫氨酸的串联酶促甲基化。
A tandem chemoenzymatic methylation by S-adenosyl-L-methionine.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0358, USA.
出版信息
Chembiochem. 2013 May 27;14(8):950-3. doi: 10.1002/cbic.201300221. Epub 2013 May 6.
Abstract
Keep 'em methylated: The in situ preparation of the cofactor AdoMet was achieved by allowing the biosynthetic enzyme SalL to operate in the reverse direction by presentation of 5'-chloro-5'-deoxyadenosine at low salt concentrations. This reaction was readily coupled with DNA and small molecule methyltransferases to afford a regioselective method for chemo-enzymatic methylation and isotope incorporation.
摘要
保持甲基化
通过在低盐浓度下提供 5'-氯-5'-脱氧腺苷，使生物合成酶 SalL 反向操作，从而实现了辅因子 AdoMet 的原位制备。该反应可与 DNA 和小分子甲基转移酶轻松偶联，提供了一种用于化学酶促甲基化和同位素掺入的区域选择性方法。
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