Günther T, Vormann J
Institute of Molecular Biology and Biochemistry, Free University of Berlin, FRG.
FEBS Lett. 1990 Jun 4;265(1-2):55-8. doi: 10.1016/0014-5793(90)80882-j.
Chicken erythrocytes preloaded with Mg2+ exchange one extracellular Mn2+ for two intracellular H+. Chicken erythrocytes preloaded with Mn2+ alone or with Mg2+ plus Mn2+ performed efflux of Mn2+, which was higher at pH 6 than at pH 7.4, indicating reversibility of Mn2+/H+ antiport. Mn2+/H+ antiport was not inhibited by 1 mM KCN plus 1 mM iodoacetic acid or 1 mM amiloride. Mn2+ influx was activated by anions. Mn2+ efflux via Mn2+/H+ antiport was inhibited by competition between H+ and K+.
预先装载Mg2+的鸡红细胞会将一个细胞外Mn2+与两个细胞内H+进行交换。单独预先装载Mn2+或同时预先装载Mg2+和Mn2+的鸡红细胞会进行Mn2+外流，其在pH 6时比在pH 7.4时更高，这表明Mn2+/H+反向转运是可逆的。Mn2+/H+反向转运不受1 mM氰化钾加1 mM碘乙酸或1 mM氨氯吡咪的抑制。Mn2+内流受阴离子激活。通过Mn2+/H+反向转运的Mn2+外流受H+和K+之间竞争的抑制。
