Structural Biophysics Research Group, School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom.
PLoS One. 2013 May 1;8(5):e63268. doi: 10.1371/journal.pone.0063268. Print 2013.
To study the structural features of human post-LASIK corneas.
A pair of post-mortem donor corneas, from a 55-year old patient who underwent uncomplicated LASIK surgery five years previously, were bisected and fixed in 4% paraformaldehyde. The right cornea and one half of the left cornea were processed for light microscopy and scanning electron microscopy. One half of the right cornea was also examined by transmission electron microscopy.
The flap-bed interface could be easily detected several years after LASIK and, although the flap appeared to be in close association with the stromal bed, there was a noticeable absence of reconnection between adjacent severed lamellae. Tissue gaps were evident at the flap margin, which once free of cellular components revealed the presence of a few bridging fibres.
Examination of corneas five years after LASIK revealed evidence of primitive reparative scar development at the wound interface, but no reconnection of severed collagen lamellae. Such findings may explain the occurrence of flap dislocation following trauma in some patients months or years after surgery.
研究人 LASIK 术后角膜的结构特征。
一对来自 55 岁患者的死后供体角膜，该患者五年前接受了无并发症的 LASIK 手术，将角膜沿水平方向切开并固定在 4%多聚甲醛中。右眼和左眼的一半角膜用于光镜和扫描电子显微镜检查。右眼的另一半角膜也进行了透射电子显微镜检查。
LASIK 术后数年，很容易就能检测到瓣床界面，尽管瓣似乎与基质床紧密相连，但相邻切断的板层之间明显没有重新连接。瓣缘处有明显的组织间隙，一旦没有细胞成分，就会发现有一些桥接纤维。
LASIK 术后五年对角膜的检查显示，在伤口界面有原始修复性瘢痕形成的证据，但切断的胶原板层没有重新连接。这些发现可能解释了一些患者在手术后数月或数年后因创伤而发生瓣移位的原因。
