Kotrashetti V S, Nayak R, Bhat K, Hosmani J, Somannavar P
Departments of Oral Pathology and Microbiology, Maratha Mandal’s NG Halgekar Institute of Dental Sciences, Belgaum 590010, Karnataka, India.
Biotech Histochem. 2013 Aug;88(6):311-22. doi: 10.3109/10520295.2013.785592. Epub 2013 May 8.
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity. TLRs are expressed in B-lymphocytes, monocytes, dendritic cells and epithelial cells. We examined the immunohistochemical expressions of TLR4 and TLR9 in various grades of oral epithelial dysplasia (OED), oral squamous cell carcinoma (OSCC) and normal oral mucosa (NOM) to determine the association between TLR4 and TLR9 in the progression of lesions from dysplasia to carcinoma. Expressions of TLR4 and TLR9 were assessed using immunohistochemistry (IHC) on paraffin embedded tissue blocks of various grades of OED (28 cases), OSCC (27 cases) and NOM (10 cases). Expression of TLR4 was high in all grades of OED and OSCC. Expression of TLR9 was high in well differentiated squamous cell carcinoma and moderately differentiated squamous cell carcinomas, and moderate to low in poorly differentiated squamous cell carcinomas. Although expression was high in case of TLR4, it was not statistically significant. Expression of TLR9 was statistically significant. In OED, expression of TLR9 was less than that of TLR4. Our results indicated that the pattern of expression of TLR4 and TLR9 increased significantly from mild to severe dysplasia compared to controls. Expression of TLR4 and TLR9 reflects progression of OED to OSCC, which suggests that TLR may play a role in tumorigenesis and that it could be used as a target for OSCC prevention and therapy in the future.
Toll样受体（TLRs）在先天免疫激活中起关键作用。TLRs在B淋巴细胞、单核细胞、树突状细胞和上皮细胞中表达。我们检测了不同分级的口腔上皮发育异常（OED）、口腔鳞状细胞癌（OSCC）和正常口腔黏膜（NOM）中TLR4和TLR9的免疫组化表达，以确定TLR4和TLR9在病变从发育异常进展为癌的过程中的关联。使用免疫组化（IHC）对不同分级的OED（28例）、OSCC（27例）和NOM（10例）的石蜡包埋组织块评估TLR4和TLR9的表达。TLR4在所有分级的OED和OSCC中表达均较高。TLR9在高分化鳞状细胞癌和中分化鳞状细胞癌中表达较高，在低分化鳞状细胞癌中表达中等至较低。虽然TLR4的表达较高，但无统计学意义。TLR9的表达具有统计学意义。在OED中，TLR9的表达低于TLR4。我们的结果表明，与对照组相比，从轻度到重度发育异常，TLR4和TLR9的表达模式显著增加。TLR4和TLR9的表达反映了OED向OSCC的进展，这表明TLR可能在肿瘤发生中起作用，并且未来可作为OSCC预防和治疗的靶点。
