Monir M M, Hiramatsu K, Yamasaki A, Nishimura K, Watanabe T
Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano, 399-4598, Japan.
Anat Histol Embryol. 2014 Apr;43(2):153-8. doi: 10.1111/ahe.12062. Epub 2013 May 7.
The influence of restricted feeding on the distribution of glucagon-like peptide-1 (GLP-1)-containing endocrine cells in the chicken small intestine was investigated using immunohistochemical and morphometrical techniques. This study demonstrated that the restricted feeding had an influence on the activity of GLP-1-immunoreactive cells in the chicken small intestine. There were differences in the localization and the frequency of occurrence of GLP-1-immunoreactive cells in the small intestine between control and restricted groups, especially 25% feed supply group provided with 25% of the intake during the adapting period. GLP-1-immunoreactive cells in the control chickens were mainly located in epithelium from crypts to the lower part of intestinal villi. Those in restricted groups, however, tended to be located from crypts to the middle part of intestinal villi. The frequency of occurrence of GLP-1-immunoreactive cells was lowest in the control group, medium in 50% feed supply group and highest in 25% feed supply group at each intestinal region examined in this study, that is, increased with the advancement of restricting the amount of feed supply. These data show that the quantity of food intake is one of signals that have an influence on the secretion of GLP-1 from L cells in the chicken small intestine.
采用免疫组织化学和形态计量学技术，研究了限饲对鸡小肠中含胰高血糖素样肽-1（GLP-1）的内分泌细胞分布的影响。本研究表明，限饲对鸡小肠中GLP-1免疫反应性细胞的活性有影响。对照组和限饲组小肠中GLP-1免疫反应性细胞的定位和出现频率存在差异，尤其是在适应期提供25%摄入量的25%饲料供应组。对照鸡的GLP-1免疫反应性细胞主要位于从隐窝到肠绒毛下部的上皮中。然而，限饲组的细胞倾向于位于从隐窝到肠绒毛中部。在本研究检测的每个肠道区域，GLP-1免疫反应性细胞的出现频率在对照组中最低，在50%饲料供应组中中等，在25%饲料供应组中最高，即随着饲料供应量限制程度的增加而增加。这些数据表明，食物摄入量是影响鸡小肠L细胞分泌GLP-1的信号之一。
