2 型糖尿病患者血清分泌型卷曲相关蛋白 5（Sfrp5）和 Wnt5a 水平。

Circulating secreted frizzled-related protein 5 (Sfrp5) and wingless-type MMTV integration site family member 5a (Wnt5a) levels in patients with type 2 diabetes mellitus.

机构信息

Division of Endocrinology and Metabolism, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan; Department of Medical Nutrition, Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan.

出版信息

Diabetes Metab Res Rev. 2013 Oct;29(7):551-6. doi: 10.1002/dmrr.2426.

DOI:10.1002/dmrr.2426

PMID:23653377

Abstract

BACKGROUND

Secreted frizzled-related protein 5 (Sfrp5), an endogenous inhibitor of wingless-type MMTV integration site family (Wnt) signalling, is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes mellitus (T2DM). Wnt member 5a (Wnt5a) is a representative ligand, and recent reports suggest that Wnt5a is involved in inflammatory diseases and metabolic disorders. The aim of this study was to investigate whether plasma Wnt5a and Sfrp5 levels are altered in patients with T2DM.

METHODS

Plasma Sfrp5 and Wnt5a concentrations were measured through enzyme-linked immunosorbent assay in type 2 diabetic and nondiabetic subjects.

RESULTS

A total of 82 patients with T2DM and 42 nondiabetic subjects were studied. Plasma Sfrp5 levels were found to be elevated in patients with T2DM (9.4 ± 9.0 vs 7.4 ± 10.9 ng/mL, p = 0.006). In contrast, Wnt5a levels were decreased (6.8 ± 12.6 vs 9.1 ± 4.0 ng/dL, p < 0.001). Increasing concentrations of Sfrp5 were independently and significantly associated with T2DM. Multiple logistic regression analysis revealed Sfrp5 as an independent association factor for T2DM, even after full adjustment of known biomarkers. In a multiple linear regression analysis, only the fasting glucose level was positively associated with the plasma Sfrp5 level.

CONCLUSIONS

Our results indicate that Sfrp5 may play a role in the pathogenesis of T2DM.

摘要

背景

分泌型卷曲相关蛋白 5（Sfrp5）是一种内源性 Wnt 信号通路抑制因子，属于抗炎脂肪因子，其在肥胖和 2 型糖尿病（T2DM）模型中表达受到干扰。Wnt 成员 5a（Wnt5a）是一种代表性配体，最近的报告表明 Wnt5a 参与了炎症性疾病和代谢紊乱。本研究旨在探讨 T2DM 患者血浆 Wnt5a 和 Sfrp5 水平是否发生改变。

方法

通过酶联免疫吸附试验测定 82 例 T2DM 患者和 42 例非糖尿病患者的血浆 Sfrp5 和 Wnt5a 浓度。

结果

共纳入 82 例 T2DM 患者和 42 例非糖尿病患者。结果发现，T2DM 患者的血浆 Sfrp5 水平升高（9.4±9.0 vs. 7.4±10.9ng/ml，p=0.006）。相反，Wnt5a 水平降低（6.8±12.6 vs. 9.1±4.0ng/dL，p<0.001）。Sfrp5 浓度的升高与 T2DM 独立且显著相关。多变量逻辑回归分析显示，Sfrp5 是 T2DM 的独立相关因素，即使在充分调整已知生物标志物后也是如此。多元线性回归分析显示，仅空腹血糖水平与血浆 Sfrp5 水平呈正相关。