Boomer Sarah M, Lodge Daniel P, Dutton Bryan E
Western Oregon University, Department of Biology, Monmouth, Oregon 97361.
Microbiol Educ. 2002 May;3:18-25. doi: 10.1128/me.3.1.18-25.2002.
We have developed a ten-week curriculum for molecular biology that uses 16S ribosomal RNA genes to characterize and compare novel bacteria from hot spring communities in Yellowstone National Park. The 16S rRNA approach bypasses selective culture-based methods. Our molecular biology course offered the opportunity for students to learn broadly applicable methods while contributing to a long-term research project. Specifically, students isolated and characterized clones that contained novel 16S rRNA inserts using restriction enzyme, DNA sequencing, and computer-based phylogenetic methods. In both classes, students retrieved novel bacterial 16S rRNA genes, several of which were most similar to Green Nonsulfur bacterial isolates. During class, we evaluated student performance and mastery of skills and concepts using quizzes, formal lab notebooks, and a broad project assignment. For this report, we also assessed student performance alongside data quality and discussed the significance, our goal being to improve both research and teaching methods.
我们开发了一门为期十周的分子生物学课程，该课程利用16S核糖体RNA基因来鉴定和比较黄石国家公园温泉群落中的新型细菌。16S rRNA方法绕过了基于选择性培养的方法。我们的分子生物学课程为学生提供了学习广泛适用方法的机会，同时也为一个长期研究项目做出了贡献。具体而言，学生们使用限制酶、DNA测序和基于计算机的系统发育方法，分离并鉴定了包含新型16S rRNA插入片段的克隆。在这两门课程中，学生们都检索到了新型细菌16S rRNA基因，其中有几个与绿色非硫细菌分离株最为相似。在课程期间，我们通过测验、正式的实验笔记本和广泛的项目作业来评估学生的表现以及他们对技能和概念的掌握程度。在本报告中，我们还将学生表现与数据质量一同进行了评估，并讨论了其意义，我们的目标是改进研究和教学方法。
