Lall Namrita, Henley-Smith Cynthia Joan, De Canha Marco Nuno, Oosthuizen Carel Basson, Berrington Danielle
Department of Plant Science, University of Pretoria, Pretoria 0002, South Africa.
Int J Microbiol. 2013;2013:420601. doi: 10.1155/2013/420601. Epub 2013 Apr 4.
This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3'-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC50) of positive drug controls on various cell lines.
本研究比较了不同的市售活力试剂。生长指示剂试剂包括碘硝基四氮唑紫（INT）、普洛麦格公司的碧色莹（PrestoBlue）和alamar蓝，它们用于对变形链球菌、中间普氏菌、痤疮丙酸杆菌和结核分枝杆菌进行抗菌分析。普洛麦格公司的碧色莹和alamar蓝是以刃天青为基础的试剂，会产生快速且易于区分的颜色变化，便于肉眼读数。INT和3'-[1-（苯基氨基羰基）-3,4-四氮唑]-双-[4-甲氧基-6-硝基]苯磺酸钠水合物（XTT）是以四氮唑为基础的试剂，在有代谢活性的线粒体酶存在的情况下会转化为甲臜染料。为了进行细胞活力分析，对试剂XTT和普洛麦格公司的碧色莹进行了比较。普洛麦格公司的碧色莹能够清楚地表明各种阳性药物对照对各种微生物菌株的最低抑菌浓度（MIC）。普洛麦格公司的碧色莹也是各种阳性药物对照对各种细胞系的50%抑制浓度（IC50）的良好指示剂。
