Koban Ina, Matthes Rutger, Hübner Nils-Olaf, Welk Alexander, Sietmann Rabea, Lademann Jürgen, Kramer Axel, Kocher Thomas
Unit of Periodontology, Dental School, University of Greifswald, Greifswald, Germany.
GMS Krankenhhyg Interdiszip. 2012;7(1):Doc06. doi: 10.3205/dgkh000190. Epub 2012 Apr 4.
Many dental diseases are attributable to biofilms. The screening of antimicrobial substances, in particular, requires a high sample throughput and a realistic model, the evaluation must be as quick and as simple as possible. For this purpose, a colorimetric assay of the tetrazolium salt XTT (sodium 3'-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) converted by saliva biofilms is recommended. Cleavage of XTT by dehydrogenase enzymes of metabolically active cells in biofilms yields a highly colored formazan product which is measured photometrically.
The suitability of the XTT assay for detecting the vitality of ex vivo saliva biofilms was tested to determine the efficacy of chlorhexidine and ozone versus saliva biofilms grown on titanium discs.
The XTT method lends itself to testing the vitality of microorganisms in saliva biofilms. The sensitivity of the arrays requires a specific minimum number of pathogens, this number being different for planktonic bacteria and those occurring in biofilms. The antibacterial effect after treatment with chlorhexidine or ozone was measured by XTT conversion that was significantly reduced. The antimicrobial efficacy of 60 s 0.5% and 0.1% chlorhexidine treatment was equal and comparable with 60 s ozone treatment.
The XTT assay is a suitable method to determine the vitality in saliva biofilms, permitting assessment of the efficacy of antimicrobial substances. Its quick and easy applicability renders it especially suitable for screening.
许多牙科疾病都归因于生物膜。尤其是抗菌物质的筛选需要高样本通量和一个逼真的模型，评估必须尽可能快速和简单。为此，推荐一种通过唾液生物膜转化的四氮唑盐XTT（3'-[1-[(苯氨基)-羰基]-3,4-四氮唑]-双(4-甲氧基-6-硝基)苯磺酸水合物钠）的比色测定法。生物膜中代谢活跃细胞的脱氢酶对XTT的裂解产生一种颜色很深的甲臜产物，该产物通过光度法进行测量。
测试了XTT测定法用于检测离体唾液生物膜活力的适用性，以确定氯己定和臭氧对在钛盘上生长的唾液生物膜的功效。
XTT方法适用于测试唾液生物膜中微生物的活力。该检测方法的灵敏度要求病原体达到特定的最小数量，这个数量对于浮游细菌和生物膜中的细菌是不同的。用氯己定或臭氧处理后的抗菌效果通过XTT转化来测量，结果显示显著降低。60秒的0.5%和0.1%氯己定处理的抗菌效果相同，且与60秒的臭氧处理相当。
XTT测定法是一种确定唾液生物膜活力的合适方法，可用于评估抗菌物质的功效。其快速且易于应用使其特别适合用于筛选。
