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宏基因组学暑期研习班:一周时间加上八名学生,产出了数十亿碱基对的克隆DNA。

Summer workshop in metagenomics: one week plus eight students equals gigabases of cloned DNA.

作者信息

Rios-Velazquez Carlos, Williamson Lynn L, Cloud-Hansen Karen A, Allen Heather K, McMahon Mathew D, Sabree Zakee L, Donato Justin J, Handelsman Jo

机构信息

Biology Department, University of Puerto Rico - Mayagüez, Mayagüez, Puerto Rico, 00681.

出版信息

J Microbiol Biol Educ. 2011 Dec 1;12(2):120-6. doi: 10.1128/jmbe.v12i2.177. Print 2011.

Abstract

We designed a week-long laboratory workshop in metagenomics for a cohort of undergraduate student researchers. During this course, students learned and utilized molecular biology and microbiology techniques to construct a metagenomic library from Puerto Rican soil. Pre-and postworkshop assessments indicated student learning gains in technical knowledge, skills, and confidence in a research environment. Postworkshop construction of additional libraries demonstrated retention of research techniques by the students.

摘要

我们为一群本科学生研究人员设计了一个为期一周的宏基因组学实验室研习班。在这个课程中,学生们学习并运用分子生物学和微生物学技术,从波多黎各土壤中构建一个宏基因组文库。研习班前和研习班后的评估表明,学生们在技术知识、技能以及在研究环境中的信心方面都有了学习收获。研习班后构建的其他文库表明学生们保留了研究技术。

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