Quak J J, van Dongen G, Koken M A, Brakkee J G, Scheper R J, Balm A J, de Jong L, Snow G B, Meijer C J
Dept. of Otolaryngology/Head and Neck Surgery, Free University Hospital, Amsterdam, The Netherlands.
Int J Cancer. 1990 Jul 15;46(1):50-5. doi: 10.1002/ijc.2910460111.
Monoclonal antibody (MAb) K 112 was generated after a single intrasplenic immunization with a recurrent laryngeal squamous-cell carcinoma. The antibody detects a 43-kDa nuclear antigen, with a pI of 5.4, which is expressed only in cycling cells. Expression is typically seen in a granular pattern excluding the nucleoli. During mitosis the bulk of the antigen is diffusely distributed in the cytoplasm. Identical reactivity was observed for tissues or cells of all mammalian species tested. These data indicate that MAb K 112 recognizes a protein belonging to the class of cell-cycle-related nuclear antigen molecules.
用复发性喉鳞状细胞癌进行脾内单次免疫后产生了单克隆抗体（MAb）K 112。该抗体可检测到一种43 kDa的核抗原，其等电点为5.4，仅在处于细胞周期的细胞中表达。表达通常呈颗粒状，不包括核仁。在有丝分裂期间，大部分抗原弥散分布于细胞质中。在所测试的所有哺乳动物物种的组织或细胞中均观察到相同的反应性。这些数据表明，MAb K 112识别一种属于细胞周期相关核抗原分子类别的蛋白质。
