Quak J J, Balm A J, van Dongen G A, Brakkee J G, Scheper R J, Snow G B, Meijer C J
Department of Otolaryngology/Head and Neck Surgery, Free University Hospital, Amsterdam, The Netherlands.
Am J Pathol. 1990 Jan;136(1):191-7.
After immunization of mice with viable cells of a metastasis of a laryngeal squamous cell carcinoma, a monoclonal antibody E 48 was obtained that detects an epitope present exclusively in squamous and transitional epithelium and their neoplastic counterparts. Immunoblotting revealed that E 48 recognizes a 22 kd molecule. Seventy-five of 76 squamous cell carcinomas from the head and neck, lung, cervix, and skin stained positively, whereas various adenocarcinomas from the colon, lung, and breast, and small cell lung carcinomas consistently stained negatively. The E 48 antigen, which is formaldehyde resistant, appears to be a reliable marker for differentiation of squamous cell carcinomas from adenocarcinoma, and small cell carcinomas.
用喉鳞状细胞癌转移灶的活细胞免疫小鼠后，获得了一种单克隆抗体E 48，它能检测到仅存在于鳞状上皮和移行上皮及其肿瘤对应物中的一个表位。免疫印迹显示E 48识别一个22kd的分子。来自头颈部、肺、宫颈和皮肤的76例鳞状细胞癌中有75例呈阳性染色，而来自结肠、肺和乳腺的各种腺癌以及小细胞肺癌始终呈阴性染色。E 48抗原对甲醛具有抗性，似乎是鳞状细胞癌与腺癌及小细胞癌鉴别的可靠标志物。
