探索基因组的三维结构：解读染色质相互作用数据。
Exploring the three-dimensional organization of genomes: interpreting chromatin interaction data.
机构信息
Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605-2324, USA.
出版信息
Nat Rev Genet. 2013 Jun;14(6):390-403. doi: 10.1038/nrg3454. Epub 2013 May 9.
Abstract
How DNA is organized in three dimensions inside the cell nucleus and how this affects the ways in which cells access, read and interpret genetic information are among the longest standing questions in cell biology. Using newly developed molecular, genomic and computational approaches based on the chromosome conformation capture technology (such as 3C, 4C, 5C and Hi-C), the spatial organization of genomes is being explored at unprecedented resolution. Interpreting the increasingly large chromatin interaction data sets is now posing novel challenges. Here we describe several types of statistical and computational approaches that have recently been developed to analyse chromatin interaction data.
摘要
DNA 在细胞核内如何三维组织，以及这如何影响细胞获取、读取和解释遗传信息的方式，是细胞生物学中最长期存在的问题之一。利用基于染色体构象捕获技术（如 3C、4C、5C 和 Hi-C）开发的新的分子、基因组和计算方法，正在以前所未有的分辨率探索基因组的空间组织。现在，解释越来越大的染色质相互作用数据集带来了新的挑战。在这里，我们描述了最近开发的几种用于分析染色质相互作用数据的统计和计算方法。
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