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基因密度、转录和绝缘子有助于将果蝇基因组分隔成物理域。

Gene density, transcription, and insulators contribute to the partition of the Drosophila genome into physical domains.

机构信息

Department of Biology, Emory University, Atlanta, GA 30322, USA.

出版信息

Mol Cell. 2012 Nov 9;48(3):471-84. doi: 10.1016/j.molcel.2012.08.031. Epub 2012 Oct 4.

DOI:10.1016/j.molcel.2012.08.031
PMID:23041285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3496039/
Abstract

The mechanisms responsible for the establishment of physical domains in metazoan chromosomes are poorly understood. Here we find that physical domains in Drosophila chromosomes are demarcated at regions of active transcription and high gene density that are enriched for transcription factors and specific combinations of insulator proteins. Physical domains contain different types of chromatin defined by the presence of specific proteins and epigenetic marks, with active chromatin preferentially located at the borders and silenced chromatin in the interior. Domain boundaries participate in long-range interactions that may contribute to the clustering of regions of active or silenced chromatin in the nucleus. Analysis of transgenes suggests that chromatin is more accessible and permissive to transcription at the borders than inside domains, independent of the presence of active or silencing histone modifications. These results suggest that the higher-order physical organization of chromatin may impose an additional level of regulation over classical epigenetic marks.

摘要

真核生物染色体物理结构域形成的机制尚未完全阐明。我们发现，果蝇染色体的物理结构域是由转录活跃和基因密度高的区域界定的，这些区域富含转录因子和特定组合的绝缘子蛋白。物理结构域包含不同类型的染色质，这些染色质由特定蛋白质和表观遗传标记的存在来定义，具有活性的染色质优先位于边界处，而沉默的染色质位于内部。结构域边界参与长距离相互作用，可能有助于活跃或沉默的染色质区域在核内聚集。转座基因分析表明，染色质在边界处比在结构域内部更易于接近和转录，而与活跃或沉默的组蛋白修饰无关。这些结果表明，染色质的高级别物理组织可能对经典表观遗传标记施加了额外的调控水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/112018824fe1/nihms-407878-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/909732c08b20/nihms-407878-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/82c529b82d07/nihms-407878-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/fa0d7d12485e/nihms-407878-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/e5cc2b5d396a/nihms-407878-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/821300c75722/nihms-407878-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/159d240b1e4e/nihms-407878-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/112018824fe1/nihms-407878-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/909732c08b20/nihms-407878-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/82c529b82d07/nihms-407878-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/fa0d7d12485e/nihms-407878-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/e5cc2b5d396a/nihms-407878-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/821300c75722/nihms-407878-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/159d240b1e4e/nihms-407878-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7aaa/3496039/112018824fe1/nihms-407878-f0007.jpg

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3
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