采用离子色谱法对人母乳中的游离硫酸盐和总磺酸酯进行定量分析。

Quantitation of free sulfate and total sulfoesters in human breast milk by ion chromatography.

作者信息

McPhee M D, Atkinson S A, Cole D E

机构信息

I.W.K. Children's Hospital, Halifax, Nova Scotia, Canada.

出版信息

J Chromatogr. 1990 Apr 27;527(1):41-50. doi: 10.1016/s0378-4347(00)82081-2.

DOI:10.1016/s0378-4347(00)82081-2

PMID:2365791

Abstract

A method for the assay of free sulfate and total sulfoesters in human breast milk by ion chromatography is described. After incubation in 1 M hydrochloric acid at 95 degrees C for 90 min, hydrolytic cleavage of sulfoester standards was essentially complete. The increase in free sulfate after hydrolysis was used as a measure of total acid-labile sulfoesters. We found that this fraction [222 +/- 16 mumol/l (mean +/- standard error), n = 29] comprised 87% of the total sulfate in mature milk. Free sulfate (35 +/- 3 mumol/l) therefore makes only a small contribution to the total sulfate pool available to human infants.

摘要

描述了一种通过离子色谱法测定人母乳中游离硫酸盐和总磺酸酯的方法。在95℃下于1M盐酸中孵育90分钟后，磺酸酯标准品的水解裂解基本完成。水解后游离硫酸盐的增加用作总酸不稳定磺酸酯的量度。我们发现该部分[222±16μmol/l（平均值±标准误），n = 29]占成熟乳中总硫酸盐的87%。因此，游离硫酸盐（35±3μmol/l）对人类婴儿可利用的总硫酸盐池的贡献很小。