Cole D E, Landry D A
J Chromatogr. 1985 Feb 8;337(2):267-78. doi: 10.1016/0378-4347(85)80040-2.
Following the previous demonstration that low concentrations of inorganic sulfate (SO4) in human serum and cerebrospinal fluid can be accurately determined by controlled-flow anion chromatography, the assay has been extended to the quantitation of free SO4 in saliva and sweat by modification of the established methods of sample collection and preparation. Salivary secretions were ultrafiltered to remove macromolecular polyanions that bind irreversibly to the anion-exchange separator column and reduce resolution. Sweat was collected from 22 fasted adult volunteers using a method which utilizes absorbent filter pads applied to the forearm after secretion had been stimulated by pilocarpine iontophoresis. It was necessary to acid wash the filter pads to reduce sulfate contamination. Saliva ultrafiltrate or sweat was diluted and injected onto a Dionex D-10 Ion Analyzer using the standard anion column system. The mean inorganic SO4 concentration in saliva from seventeen adult fasting volunteers was 72 +/- 4 mumol/l (+/- S.E.); the mean SO4 concentration in sweat was 83 +/- 3 mumol/l. Both are significantly less than in matching serum, suggesting that SO4 is actively removed during formation of these glandular secretions. The ion chromatographic assay is shown to be capable of measuring SO4 in biological fluids at concentrations that are otherwise undetectable by conventional assay techniques.
先前的研究表明，通过控制流量阴离子色谱法可以准确测定人血清和脑脊液中低浓度的无机硫酸盐（SO4）。在此基础上，通过改进已有的样品采集和制备方法，该检测方法已扩展到唾液和汗液中游离SO4的定量分析。对唾液分泌物进行超滤，以去除与阴离子交换分离柱不可逆结合并降低分离度的大分子聚阴离子。采用毛果芸香碱离子导入法刺激分泌后，利用贴在前臂上的吸收性滤垫从22名禁食成年志愿者身上收集汗液。有必要对滤垫进行酸洗以减少硫酸盐污染。将唾液超滤物或汗液稀释后，使用标准阴离子柱系统注入戴安D-10离子分析仪。17名成年禁食志愿者唾液中的平均无机SO4浓度为72±4μmol/L（±标准误）；汗液中的平均SO4浓度为83±3μmol/L。两者均显著低于匹配的血清中的浓度，这表明在这些腺体分泌物形成过程中SO4被主动清除。离子色谱检测方法能够测量生物体液中常规检测技术无法检测到浓度的SO4。
