Cole D E, Evrovski J
Department of Laboratory Medicine, Banting Institute, University of Toronto, Ontario, Canada.
J Chromatogr A. 1997 Nov 21;789(1-2):221-32. doi: 10.1016/s0021-9673(97)00821-2.
For assay of serum sulfate, quantitation by ion conductimetry after separation by anion-exchange chromatography is the method of choice. In comparison to classical barium precipitation methods, chromatographic methods demonstrate increased precision, specificity and sensitivity, and they may be superior to spectrophotometric methods that rely on organic cation precipitation of sulfate. The increased sensitivity and specificity, as well as the inherent capacity of chromatographic methods for simultaneous determination of other anions, has led to its increasing use in the determination of excreted sulfate in clinical profiles of urinary anion composition. Ion chromatography can also be used to quantitate free sulfate in other clinical samples, including cerebrospinal fluid, sweat, saliva, breast milk and human tissues. Finally, ion chromatography shows promise as a more precise and sensitive method for measurement of total acid-labile sulfoesters and thiosulfate.
对于血清硫酸盐的测定，阴离子交换色谱分离后通过离子电导法进行定量是首选方法。与传统的钡沉淀法相比，色谱法具有更高的精密度、特异性和灵敏度，并且可能优于依赖硫酸盐有机阳离子沉淀的分光光度法。灵敏度和特异性的提高，以及色谱法同时测定其他阴离子的固有能力，使其在尿阴离子组成临床分析中排泄硫酸盐的测定中得到越来越多的应用。离子色谱还可用于定量其他临床样品中的游离硫酸盐，包括脑脊液、汗液、唾液、母乳和人体组织。最后，离子色谱有望成为一种更精确、灵敏的方法来测量总酸不稳定硫酯和硫代硫酸盐。
