National Centre for Cell Science, Ganeshkhind, Pune 411 007, India.
J Biosci. 2013 Jun;38(2):301-9. doi: 10.1007/s12038-013-9320-0.
Germ band retraction (GBR) stage is one of the important stages during insect development. It is associated with an extensive epithelial morphogenesis and may also be pivotal in generation of morphological diversity in insects. Despite its importance, only a handful of studies report the transcriptome repertoire of this stage in insects. Here, we report generation, annotation and analysis of ESTs from the embryonic stage (16-22 h post fertilization) of laboratoryreared Anopheles stephensi mosquitoes. A total of 1002 contigs were obtained upon clustering of 1140 high-quality ESTs, which demonstrates an astonishingly low transcript redundancy (12.1 percent). Putative functions were assigned only to 213 contigs (21 percent), comprising mainly of transcripts encoding protein synthesis machinery. Approximately 78 percent of the transcripts remain uncharacterized, illustrating a lack of sequence information about the genes expressed in the embryonic stages of mosquitoes. This study highlights several novel transcripts, which apart from insect development, may significantly contribute to the essential biological complexity underlying insect viability in adverse environments. Nonetheless, the generated sequence information from this work provides a comprehensive resource for genome annotation, microarray development, phylogenetic analysis and other molecular biology applications in entomology.
胚带回缩（GBR）阶段是昆虫发育过程中的重要阶段之一。它与广泛的上皮形态发生有关，也可能是昆虫形态多样性产生的关键。尽管它很重要，但只有少数研究报告了昆虫这一阶段的转录组谱。在这里，我们报告了实验室饲养的致倦库蚊胚胎期（受精后 16-22 小时）的 EST 的生成、注释和分析。在对 1140 个高质量 EST 进行聚类后，获得了总共 1002 个连续序列，这表明转录本的冗余度极低（12.1%）。仅为 213 个连续序列（21%）分配了推定功能，主要包括编码蛋白质合成机制的转录本。大约 78%的转录本尚未被描述，说明关于蚊子胚胎阶段表达的基因的序列信息缺乏。这项研究突出了几个新的转录本，除了昆虫发育之外，它们可能对昆虫在不利环境中的生存所必需的生物复杂性有重要贡献。尽管如此，这项工作产生的序列信息为基因组注释、微阵列开发、系统发育分析和昆虫学中的其他分子生物学应用提供了一个全面的资源。
