布鲁氏菌 O 抗原结构的再研究。
Reinvestigation of the structure of Brucella O-antigens.
机构信息
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
出版信息
Carbohydr Res. 2013 Aug 30;378:144-7. doi: 10.1016/j.carres.2013.03.021. Epub 2013 Apr 6.
O-Specific polysaccharides of Brucella contain two antigenic determinants, called A and M. Most of the strains express epitope A with a small amount of epitope M, whereas Brucella melitensis strain 16 M expresses longer polymer consisting mostly of M-type epitopes. Proposed explanation was that epitope A is defined by 1-2-linked homopolymer of N-formylperosamine (Rha4NFo), while epitope M is a pentasaccharide with four 2- and one 3-substituted Rha4NFo. We reinvestigated both types of structures by 2D NMR and showed that M-epitope is a tetrasaccharide, missing one of the 2-linked Rha4NFo as compared to the previously proposed structure. Polysaccharide from B. melitensis 16 M contains a fragment of 1-2-linked polymer, capped with M-type polymer. Other strains contain one or two M-type units at the non-reducing end of the 1-2-linked O-chain.
布鲁氏菌 O-特异性多糖含有两个抗原决定簇，称为 A 和 M。大多数菌株表达带有少量 M 表位的 A 表位，而布鲁氏菌 melitensis 16 M 株则表达由大多数 M 型表位组成的较长聚合物。提出的解释是，A 表位由 N-甲酰基-4-去氨基-2,3,4-三脱氧-D-赤己糖（Rha4NFo）的 1-2 连接同聚物定义，而 M 表位是一个五糖，具有四个 2-和一个 3-取代的 Rha4NFo。我们通过 2D NMR 重新研究了这两种结构，并表明与之前提出的结构相比，M 表位是一个四糖，缺少一个 2-连接的 Rha4NFo。来自 B. melitensis 16 M 的多糖含有 1-2 连接聚合物的片段，其末端带有 M 型聚合物。其他菌株在 1-2 连接 O-链的非还原端含有一个或两个 M 型单元。
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