The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, United Kingdom.
J Immunol. 2013 Jun 15;190(12):6389-96. doi: 10.4049/jimmunol.1300365. Epub 2013 May 10.
Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14(hi)-CD163(low) and CD14(low)-CD163(hi), show approximately equal abundance in the steady-state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human-specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14(low)-CD163(high) cells are actually equivalent to intermediate human monocytes, and there is no CD14(-) CD16(+) "nonclassical" population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function.
基于表面标志物的表达，人类和小鼠单核细胞可分为两个不同的亚群：CD14/16 和 Ly6C/CX3CR1。猪单核细胞亚群是根据 CD14 和清道夫受体 CD163 的相互表达来鉴定的。这两个群体，CD14(hi)-CD163(low)和 CD14(low)-CD163(hi)，在稳态下的丰度大致相等。CSF1 培养猪 PBMC 表明，这两个群体是由这种生长因子控制的成熟系列。使用新开发的注释猪全基因组雪球微阵列对猪单核细胞亚群的基因表达进行了分析。先前的研究表明，人类和小鼠亚群之间存在功能等效性，但某些基因，如 CD36、CLEC4E 或 TREM-1，表现出人类特异性表达。相同的基因选择性地在猪单核细胞亚群中表达。然而，这些图谱表明，猪 CD14(low)-CD163(high)细胞实际上相当于中间人类单核细胞，并且没有 CD14(-) CD16(+)“非经典”群体。这些结果从理解人类单核细胞功能的角度讨论了猪作为模型的相关性。
