Laboratory of Exercise Physiology, Department of Physical Education, College of Sports Science, Dong-A University, Busan, Republic of Korea.
Biochem Biophys Res Commun. 2013 Jun 7;435(3):454-9. doi: 10.1016/j.bbrc.2013.05.007. Epub 2013 May 11.
The present study examined the change to the effect of the leptin sensitivity by leptin resistance-induced leptin receptor (ObRb) and leptin-related suppressor of cytokine signaling 3 (SOCS3) mRNA levels in hypothalamic, liver, muscle and leptin protein levels in blood after eight 8 weeks of exercise training and/or dietary control of high fat induced obese rats. After 2 weeks of adaptation maintenance, four-week-old male SD rats (n=42) were randomly divided into control (CO) (n=8) and high-fat diet (HF) (n=32) groups. The HF group randomly divided into HF, HF+exercise training (HFT), changed to normal diet (HFND) and changed to normal diet and exercise training (HFNDT) groups. 13 weeks of HF group average body weight significantly increased in comparison to the CO group (p<0.05). Plasma leptin levels of the HFT, HFND and HFNDT group were significantly decreased in comparison to the HF group (p<0.05). The mRNA expression of ObRb and SOCS3 in the liver and muscle of the HF group was significantly decreased comparison to that of the HFT, HFND and HFNDT group after 8 weeks intervention (p<0.05). In addition, the mRNA expression of ObRb and SOCS3 in the hypothalamus of the HF group was significantly increased comparison to that of the HFT, HFND and HFNDT group (p<0.05). HFND group also was significantly reduced comparison to of the HFT and HFNDT group (p<0.05). These findings suggest that the effect of leptin sensitivity in peripheral may primarily the more relate to combined dietary control and exercise training more than effect of dietary control.
本研究旨在探讨肥胖诱导的高脂肪饮食（HF）大鼠经过 8 周的运动训练和/或饮食控制后，瘦素受体（ObRb）和瘦素相关抑制因子 3（SOCS3）mRNA 水平在大脑下丘脑、肝脏、肌肉中的变化以及血液中瘦素蛋白水平的变化。
适应性维持 2 周后，将 4 周龄雄性 SD 大鼠（n=42）随机分为对照组（CO）（n=8）和高脂肪饮食组（HF）（n=32）。HF 组再随机分为 HF、HF+运动训练（HFT）、改为正常饮食（HFND）和改为正常饮食和运动训练（HFNDT）组。13 周后，HF 组大鼠平均体重显著高于 CO 组（p<0.05）。与 HF 组相比，HFT、HFND 和 HFNDT 组的血浆瘦素水平显著降低（p<0.05）。8 周干预后，HF 组肝脏和肌肉中 ObRb 和 SOCS3 的 mRNA 表达显著低于 HFT、HFND 和 HFNDT 组（p<0.05）。此外，与 HFT、HFND 和 HFNDT 组相比，HF 组下丘脑 ObRb 和 SOCS3 的 mRNA 表达显著增加（p<0.05）。HFND 组与 HFT 和 HFNDT 组相比也显著降低（p<0.05）。这些发现表明，外周组织中瘦素敏感性的变化主要与饮食控制和运动训练的联合作用有关，而不是饮食控制的影响。
