School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian, Liaoning 116023, China.
Bioresour Technol. 2013 Jul;140:15-21. doi: 10.1016/j.biortech.2013.03.184. Epub 2013 Apr 6.
Using pyrene as a sole carbon, a new polycyclic aromatic hydrocarbons (PAHs)-degrading bacterial strain was isolated from the active sewage sludge. This strain was identified as Pseudomonas sp. Jpyr-1 by 16S rRNA gene sequence analysis. The maximum degradation rate of pyrene was 3.07 mg L(-1)h(-1) in 48 h incubation with initial pyrene concentration of 200 mg L(-1). Moreover, in binary system consisting of pyrene and another PAH, the enzyme system of Jpyr-1 showed a preference toward pyrene. Furthermore, competitive inhibition of pyrene degradation by other PAH compounds occurred in the binary system. Jpyr-1 could also rapidly degrade other PAHs, such as benzanthracene, chrysene and benzo[a]pyrene. Moreover, several metabolites were detected during pyrene degradation which indicated that Jpyr-1 degraded pyrene through the o-phthalate pathway. Taken together, these results indicated that Pseudomonas sp. Jpyr-1 was a new PAHs-degrading strain that might be useful in the bioremediation of sites contaminated with PAHs.
从活性污泥中分离到一株以芘为唯一碳源的多环芳烃（PAHs）降解细菌，通过 16S rRNA 基因序列分析，鉴定该菌株为假单胞菌属 Jpyr-1。在初始芘浓度为 200mg/L 时，该菌在 48h 内对芘的最大降解率为 3.07mg/L/h。此外，在芘和另一种 PAH 的二元体系中，Jpyr-1 的酶系表现出对芘的偏好。此外，在二元体系中，其他 PAH 化合物对芘降解存在竞争抑制作用。Jpyr-1 还可以快速降解其他 PAHs，如苯并蒽、屈和苯并[a]芘。此外，在芘降解过程中检测到几种代谢产物，这表明 Jpyr-1 通过邻苯二甲酸途径降解芘。综上所述，这些结果表明，假单胞菌属 Jpyr-1 是一株新的 PAHs 降解菌，可能在 PAHs 污染场地的生物修复中具有应用价值。
