Sancilio Silvia, di Giacomo Viviana, Di Giulio Mara, Gallorini Marialucia, Marsich Eleonora, Travan Andrea, Tarusha Lorena, Cellini Luigina, Cataldi Amelia
Department of Pharmacy, "G. d'Annunzio" University, Chieti-Pescara, Italy.
Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.
PLoS One. 2014 May 7;9(5):e96520. doi: 10.1371/journal.pone.0096520. eCollection 2014.
This study sought to evaluate the in vitro biological response of human gingival fibroblasts (HGFs) co-coltured with Streptococcus mitis to bisphenol A glycidylmethacrylate/triethylene glycol dimethacrylate (BisGMA/TEGDMA) thermosets coated with Chitlac-nAg, a nanocomposite system with antimicrobial properties. To avoid bacterial adhesion to dental devices and to reduce cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilizing silver nanoparticles, which have well-known antimicrobial properties, with a polyelectrolyte solution containing Chitlac. Cytotoxicity, cell morphology, cell migration and inflammatory interleukine-6 (IL-6) and prostaglandin E2 (PGE2) secretion were evaluated. Our results showed that the cytotoxicity exerted on HGFs by our nanocomposite material was absent in our co-culture model, where fibroblasts are able to adhere and migrate. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release rises up 20%. Moreover the presence of S. mitis reduced this release in a greater amount with Chitlac-nAg coated thermosets. The secretion of IL-6 was significant in both Chitlac and Chitlac-nAg coated thermosets, but PGE2 production was minimal, suggesting that the IL-6 production was not related to an inflammatory response. Co-culture and the addiction of saliva did not influence IL-6 and PGE2 secretion. Data obtained in the present work suggest that Chitlac n-Ag coated thermosets could significantly improve the success rates of restorative dentistry, since they limit bacterial adhesion and are not toxic to HGFs.
本研究旨在评估与缓症链球菌共培养的人牙龈成纤维细胞（HGFs）对涂有Chitlac-nAg的双酚A甲基丙烯酸缩水甘油酯/三乙二醇二甲基丙烯酸酯（BisGMA/TEGDMA）热固性材料的体外生物学反应，Chitlac-nAg是一种具有抗菌特性的纳米复合体系。为避免细菌粘附于牙科器械并降低对真核细胞的细胞毒性，我们用一种新材料Chitlac-nAg涂覆BisGMA/TEGDMA甲基丙烯酸热固性材料，该材料通过用含有Chitlac的聚电解质溶液稳定具有众所周知抗菌特性的银纳米颗粒而形成。评估了细胞毒性、细胞形态、细胞迁移以及炎性白细胞介素-6（IL-6）和前列腺素E2（PGE2）的分泌。我们的结果表明，在成纤维细胞能够粘附和迁移的共培养模型中，我们的纳米复合材料对HGFs没有细胞毒性。24小时后，涂有Chitlac以及涂有Chitlac-nAg的热固性材料对HGFs产生的细胞毒性最小，而48小时后乳酸脱氢酶（LDH）释放量上升20%。此外，缓症链球菌的存在使涂有Chitlac-nAg的热固性材料的这种释放量减少得更多。IL-6在涂有Chitlac和Chitlac-nAg的热固性材料中分泌均显著，但PGE2产生量最小，这表明IL-6的产生与炎症反应无关。共培养和添加唾液均不影响IL-6和PGE2的分泌。本研究获得的数据表明，涂有Chitlac n-Ag的热固性材料可显著提高修复牙科的成功率，因为它们限制细菌粘附且对HGFs无毒。
