Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul 143‑701, Republic of Korea.
Mol Med Rep. 2013 Jul;8(1):183-8. doi: 10.3892/mmr.2013.1469. Epub 2013 May 10.
Endometriosis is a gynecological disease defined as the presence of endometrial tissue outside the uterine cavity, which is caused by various factors. Proteomic analysis of two sets of eutopic endometrial cells collected from the menstrual blood of females with (n=6; n=3) or without (n=6; n=3) endometriosis was performed to identify novel potential biomarkers for endometriosis. The data revealed that samples from endometriosis patients had stem cell characteristics, as they had higher mRNA expression levels of octamer-binding transcription factor 4 (Oct-4), C-X-C chemokine receptor type 4 (CXCR4), SRY-box containing gene 2 (SOX2) and mesenchymal-epithelial transition factor (MET) compared with that of the normal controls. Three proteins, collapsin response mediator protein 2 (CRMP2), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) and myosin regulatory light polypeptide 9 (MYL9), were simultaneously identified from the two sets of samples from females with or without endometriosis by two-dimensional electrophoresis (2-DE). A difference in CRMP2 expression was confirmed with western blotting. Taken together, the results suggest that CRMP2 plays a role in the pathogenesis of endometriosis.
子宫内膜异位症是一种妇科疾病，定义为子宫内膜组织出现在子宫腔外，其由多种因素引起。为了鉴定子宫内膜异位症的新型潜在生物标志物，我们对两组取自月经血中的在位子宫内膜细胞进行了蛋白质组学分析，这些细胞来源于患有子宫内膜异位症的女性（n=6；n=3）或不患有子宫内膜异位症的女性（n=6；n=3）。数据显示，与正常对照组相比，来自子宫内膜异位症患者的样本具有干细胞特征，因为它们的八聚体结合转录因子 4（Oct-4）、C-X-C 趋化因子受体 4（CXCR4）、SRY 框基因 2（SOX2）和间充质上皮转化因子（MET）的 mRNA 表达水平更高。通过二维电泳（2-DE），我们从患有或不患有子宫内膜异位症的两组女性的样本中同时鉴定到了 3 种蛋白质，即 collapsin 反应介质蛋白 2（CRMP2）、泛素羧基末端水解酶同工酶 L1（UCH-L1）和肌球蛋白调节轻链 9（MYL9）。Western blot 验证了 CRMP2 表达的差异。综上所述，结果表明 CRMP2 在子宫内膜异位症的发病机制中发挥作用。
