Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
Int J Mol Med. 2013 Dec;32(6):1394-400. doi: 10.3892/ijmm.2013.1506. Epub 2013 Sep 24.
Endometriosis, characterized by the growth of the endometrial gland and stroma outside the uterine cavity, is a gynecological disorder affecting 6‑10% of women of reproductive age. However, the pathogenesis of endometriosis and the molecular mechanisms involved in the progression of this disease remain to be clarified. Therefore, in this study two-dimensional gel electrophoresis (2‑DE) combined with mass spectrometry (MS) were applied to explore endometrial proteins with a role in the progression of endometriosis. Expression of global proteins in ectopic endometrial tissue (n=13; endometriosis group) was compared with that of the normal endometrial tissue (n=6; control group). Sixteen differently expressed proteins, including Vitamin D binding protein (DBP), with various functions were primarily identified in the ectopic endometrial tissue. DBP was confirmed to be significantly increased in the ectopic endometrial tissue compared with that in the normal endometrial tissue (P<0.05). Results of the present study therefore showed that DBP may play an important role in the progression of endometriosis.
子宫内膜异位症是一种妇科疾病，其特征是子宫内膜腺体和基质在子宫腔外生长，影响 6-10%的育龄妇女。然而，子宫内膜异位症的发病机制以及该疾病进展中涉及的分子机制仍有待阐明。因此，在这项研究中，二维凝胶电泳（2-DE）结合质谱（MS）技术被应用于探索与子宫内膜异位症进展相关的子宫内膜蛋白。比较了异位子宫内膜组织（n=13；子宫内膜异位症组）和正常子宫内膜组织（n=6；对照组）中全局蛋白质的表达。在异位子宫内膜组织中主要鉴定出了 16 种具有不同功能的差异表达蛋白，包括维生素 D 结合蛋白（DBP）。与正常子宫内膜组织相比，DBP 在异位子宫内膜组织中明显增加（P<0.05）。因此，本研究结果表明 DBP 可能在子宫内膜异位症的进展中发挥重要作用。
