Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha 410081, People's Republic of China.
J Biochem Mol Toxicol. 2013 Jul;27(7):337-42. doi: 10.1002/jbt.21493. Epub 2013 May 13.
Up to now, there have been a few reports on the toxic components purified from black widow spider (Latrodectus tredecimguttatus) eggs. In the present study, a novel neurotoxic protein was purified from the eggs by gel filtration combined with ion-exchange chromatography. Its molecular weight was 23.752 kDa determined by electrospray mass spectrometry. The protein could block the neuromuscular transmission in mouse-isolated phrenic nerve-hemidiaphragm preparations completely in a reversible manner and activate tetrodotoxin-sensitive sodium current in rat dorsal root ganglion cells. The N-terminal sequence of the protein was identified by the Edman degradation to be N-S-I-A-D-D-R-Y-R-W-P-G-Y-P-G-A-G-L-I-P-Y-I-I-D-S-. When the sequence was used to search against protein database with a sequence query in Mascot engine there was no matched sequence or protein whereas the Basic Local Alignment Search Tool (BLAST) analysis indicated that no significant similarity was found. These results demonstrated that the protein (named Latroeggtoxin-I) is a novel neurotoxic protein purified from the eggs of black widow spiders.
迄今为止，已有几篇关于从黑寡妇蜘蛛（Latrodectus tredecimguttatus）卵中纯化出的有毒成分的报告。本研究通过凝胶过滤结合离子交换层析从卵中纯化出一种新型的神经毒性蛋白。电喷雾质谱法测定其分子量为 23.752 kDa。该蛋白可完全可逆地阻断小鼠膈神经-膈肌分离标本中的神经肌肉传递，并激活河豚毒素敏感的大鼠背根神经节细胞钠电流。该蛋白的 N 端序列通过 Edman 降解鉴定为 N-S-I-A-D-D-R-Y-R-W-P-G-Y-P-G-A-G-L-I-P-Y-I-I-D-S-。当该序列用于 Mascot 引擎中的序列查询进行蛋白质数据库搜索时，没有匹配的序列或蛋白质，而基本局部比对搜索工具（BLAST）分析表明没有发现显著的相似性。这些结果表明，该蛋白（命名为 Latroeggtoxin-I）是从黑寡妇蜘蛛卵中纯化出的一种新型神经毒性蛋白。
