Department of Health and Exercise Science, Colorado State University, Fort Collins, CO 8052, USA.
Diabetol Metab Syndr. 2013 May 14;5(1):24. doi: 10.1186/1758-5996-5-24.
Lipidomic analysis was performed to explore differences in lipid profiles between plasma from lean and obese subjects, followed by in vitro methods to examine a role for the identified lipids in endothelial cell pathophysiology.
Plasma was collected from 15 morbidly obese and 13 control subjects. Lipids were extracted from plasma and analyzed using LC/MS, and MS/MS to characterize lipid profiles and identify lipids that are elevated in obese subjects compared to lean.
Orthogonal partial least squares-discriminant analysis (OPLS-DA) modelling showed that lipid profiles were significantly different in obese subjects compared to lean. Analysis of lipids that were driving group separation in the OPLS-DA model and that were significantly elevated in the obese group led to identification of a group of ether-linked phosphatidylcholine (PC) and phosphatidylethanolamine (PE) lipids of interest. Treatment of human coronary artery endothelial cells with the ether-linked phosphatidylethanolamine induced expression of cell adhesion molecules, a hallmark of endothelial cell activation. However, oxidized phosphatidylcholine products that can induce endothelial cell activation in vitro, were not significantly different between groups in vivo.
These data suggest a role for ether-linked lipids in obesity associated dyslipidemia and vascular disease.
进行脂质组学分析以探讨肥胖和非肥胖个体血浆中脂质谱的差异，然后采用体外方法研究鉴定出的脂质在血管内皮细胞病理生理学中的作用。
从 15 名病态肥胖和 13 名对照受试者中采集血浆。从血浆中提取脂质，并用 LC/MS 和 MS/MS 进行分析，以描述脂质谱并鉴定与肥胖个体相比在肥胖个体中升高的脂质。
正交偏最小二乘判别分析（OPLS-DA）模型显示，肥胖个体的脂质谱与非肥胖个体有显著差异。对 OPLS-DA 模型中驱动分组分离的脂质和肥胖组中显著升高的脂质进行分析，鉴定出一组醚键连接的磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）脂质。用醚键连接的磷脂酰乙醇胺处理人冠状动脉内皮细胞可诱导细胞黏附分子表达，这是内皮细胞激活的标志。然而，体外可诱导内皮细胞激活的氧化磷脂酰胆碱产物在体内各组间无显著差异。
这些数据表明，醚键连接的脂质在肥胖相关的血脂异常和血管疾病中起作用。
