Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA.
Gut Microbes. 2013 Jul-Aug;4(4):347-52. doi: 10.4161/gmic.24827. Epub 2013 May 2.
Human gastrointestinal microbial communities are recognized as important determinants of the host health and disease status. We have recently examined the distal gut microbiota of two groups of children: healthy adolescents and those diagnosed with diarrhea-predominant irritable bowel syndrome (IBS). We have revealed the common core of phylotypes shared among all children, identified genera differentially abundant between two groups and surveyed possible relationships among intestinal microbial genera and phylotypes. In this article we explored the use of supervised and unsupervised ordination and classification methods to separate and classify child fecal samples based on their quantitative microbial profile. We observed sample separation according to the participant health status, and this separation could often be attributed to the abundance levels of several specific microbial genera. We also extended our original correlation network analysis of the relative abundances of bacterial genera across samples and determined possible association networks separately for healthy and IBS groups. Interestingly, the number of significant genus abundance associations was drastically lower among the IBS samples, which can potentially be attributed to the existence of multiple routes to microbiota disbalance in IBS or to the loss of microbial interactions during IBS development.
人类胃肠道微生物群落被认为是宿主健康和疾病状况的重要决定因素。我们最近研究了两组儿童的远端肠道微生物群：健康青少年和被诊断为腹泻型肠易激综合征（IBS）的儿童。我们揭示了所有儿童共有的分类群的共同核心，确定了两组之间丰度不同的属，并调查了肠道微生物属和分类群之间的可能关系。在本文中，我们探索了使用有监督和无监督排序和分类方法，根据微生物定量特征分离和分类儿童粪便样本。我们观察到根据参与者健康状况的样本分离，这种分离通常可以归因于几个特定微生物属的丰度水平。我们还扩展了我们对细菌属相对丰度的原始相关网络分析，并分别为健康和 IBS 组确定了可能的关联网络。有趣的是，IBS 样本中显著的属丰度关联数量明显减少，这可能归因于 IBS 中存在多种微生物失衡的途径，或者在 IBS 发展过程中微生物相互作用的丧失。
