Sotgiu S, Arru G, Fois M L, Sanna A, Musumeci M, Rosati G, Musumeci S
Institute of Clinical Neurology, University of Sassari, Sassari, Italy;
Int J Biomed Sci. 2006 Jun;2(2):114-20.
The 1,2-fucosyl-oligosaccharides, and among these the 2'-fucosyl-lactose (2'-FL) and lacto-N-fucopentaose (LNFP)-I, are quantitatively the most represented oligosaccharides of human milk. They are also seen to represent an important immune device to prevent nursing infants from severe infectious diarrhoea. Recent evidences show that the appearance of 2'-FL and LNFP-I in human colostrums is synchronised with the macrophage inhibition and that LNFP-III induces a Th2 response from the mouse peripheral immune system. Since mannosyl-fucosyl receptors are described on the macrophage surface, all these evidences allow us to investigate on the possible immune function of human 2'-FL and LNFP-I in vitro on LPS-activated mononuclear cells (MNC) from 12 patients with multiple sclerosis (MS) and 20 matched health controls (HC). We found that 2'-FL and LNFP-I significantly decrease, to a different extent, the MNC proliferation from both HC and MS patients, in a linear and dose-dependent manner. 2'-FL and LNFP-I also reduce the production of IL-12 and IFN-γ, particularly in MS patients as compared to HC (p=0.01 and p<0.001, respectively), while increasing that of IL-10. The overall immunomodulatory effect of 2'-FL and LNFP I here presented may represent a future therapeutic option for the abnormal immune response found in some monocyte-mediated diseases.
1,2-岩藻糖基寡糖，其中2'-岩藻糖基乳糖（2'-FL）和乳糖-N-岩藻五糖（LNFP）-I，在人乳中是含量最丰富的寡糖。它们也是预防哺乳婴儿严重感染性腹泻的重要免疫机制。最近的证据表明，人初乳中2'-FL和LNFP-I的出现与巨噬细胞抑制同步，并且LNFP-III可诱导小鼠外周免疫系统产生Th2反应。由于在巨噬细胞表面发现了甘露糖基-岩藻糖基受体，所有这些证据使我们能够在体外研究人2'-FL和LNFP-I对12例多发性硬化症（MS）患者和20例匹配的健康对照（HC）的脂多糖激活的单核细胞（MNC）的可能免疫功能。我们发现，2'-FL和LNFP-I以线性和剂量依赖性方式不同程度地显著降低HC和MS患者的MNC增殖。2'-FL和LNFP-I还降低IL-12和IFN-γ的产生，特别是与HC相比，MS患者中降低更明显（分别为p = 0.01和p <0.001），同时增加IL-10的产生。这里呈现的2'-FL和LNFP I的整体免疫调节作用可能代表了一些单核细胞介导疾病中异常免疫反应的未来治疗选择。
